Recombinant Human IFT81 Protein, N-His

Introduction to Recombinant Human IFT81 Protein

Recombinant Human IFT81 Protein is a key protein involved in the process of intraflagellar transport (IFT) in eukaryotic cells. This protein is encoded by the IFT81 gene and is a part of the IFT-B complex, which is responsible for the movement of proteins and other molecules along the cilia and flagella of cells. Recombinant Human IFT81 Protein is produced through recombinant DNA technology and is widely used in various research and medical applications.

Structure of Recombinant Human IFT81 Protein

The IFT81 gene is located on chromosome 22 in humans and is composed of 11 exons. The protein product of this gene, Recombinant Human IFT81 Protein, is a 781 amino acid long polypeptide with a molecular weight of approximately 88 kDa. It contains several structural domains, including a coiled-coil domain, a WD40 repeat domain, and a C-terminal domain. These domains are essential for the proper functioning of the protein in the IFT-B complex.

Recombinant Human IFT81 Protein has a conserved structure across different species, indicating its vital role in the IFT process. It forms a complex with other IFT-B proteins, including IFT74, IFT52, and IFT46, to facilitate the transport of cargo along the ciliary and flagellar axoneme. The structural integrity of this complex is crucial for the proper functioning of cilia and flagella, which are essential for various cellular processes, including sensing, motility, and signaling.

Activity of Recombinant Human IFT81 Protein

Recombinant Human IFT81 Protein plays a crucial role in the process of intraflagellar transport, which is essential for the assembly and maintenance of cilia and flagella. It acts as a molecular motor, using ATP hydrolysis to power the movement of proteins and other molecules along the axoneme. This transport process is crucial for the proper functioning of cilia and flagella, as well as for the cell’s overall health and survival.

Moreover, Recombinant Human IFT81 Protein is involved in the regulation of ciliary length and the transport of signaling molecules, such as G protein-coupled receptors (GPCRs), to the ciliary membrane. It also plays a role in the trafficking of specific proteins and lipids to and from the cilia, which is essential for maintaining the cell’s homeostasis and response to environmental stimuli.

Applications of Recombinant Human IFT81 Protein

Recombinant Human IFT81 Protein has a wide range of applications in both research and medical fields. It is commonly used as an antigen in studies related to cilia and flagella, as well as in the development of therapeutic agents targeting ciliary dysfunction. Its role in the transport of GPCRs makes it a potential target for the treatment of various diseases, including polycystic kidney disease, retinal degeneration, and obesity.

Furthermore, Recombinant Human IFT81 Protein is used in the production of diagnostic assays for ciliary disorders. It can also be used for the purification and characterization of other IFT-B complex proteins, which can aid in understanding their role in ciliary function and dysfunction.

Conclusion

In summary, Recombinant Human IFT81 Protein is a crucial protein involved in the process of intraflagellar transport. It plays a vital role in the assembly, maintenance, and function of cilia and flagella, which are essential for various cellular processes. Its production through recombinant DNA technology has enabled its widespread use in research and medical applications, making it an essential tool in understanding and treating ciliary dysfunction.