Recombinant Human ELAVL1 Protein, N-His

Introduction

Recombinant Human ELAVL1 Protein, also known as HuR, is a highly conserved RNA-binding protein that plays a crucial role in regulating gene expression. It is encoded by the ELAVL1 gene and is expressed in various tissues, including the brain, heart, and liver. This protein has been extensively studied and has been found to have important functions in cell proliferation, differentiation, and survival. In this article, we will discuss the structure, activity, and applications of Recombinant Human ELAVL1 Protein.

Structure of Recombinant Human ELAVL1 Protein

Recombinant Human ELAVL1 Protein is a 326 amino acid protein with a predicted molecular weight of 36.6 kDa. It contains three RNA recognition motifs (RRMs) and a nuclear localization signal (NLS) at the C-terminus. The RRMs are responsible for binding to specific RNA sequences, while the NLS allows for the protein to enter the nucleus where it carries out its functions.

The crystal structure of Recombinant Human ELAVL1 Protein has been determined, revealing a compact globular structure with the three RRMs arranged in a linear fashion. The RRMs are connected by flexible linkers, allowing for conformational changes that are necessary for RNA binding. The NLS is located at the end of the third RRM and is essential for the nuclear localization of the protein.

Activity of Recombinant Human ELAVL1 Protein

Recombinant Human ELAVL1 Protein is a multifunctional protein that regulates gene expression at the post-transcriptional level. It binds to specific AU-rich elements (AREs) in the 3’ untranslated region (UTR) of target mRNAs, stabilizing them and increasing their translation. This leads to an increase in the levels of proteins involved in cell proliferation, differentiation, and survival.

In addition to its role in RNA stabilization, Recombinant Human ELAVL1 Protein also regulates alternative splicing of target mRNAs. By binding to specific sequences in the pre-mRNA, it can promote or inhibit the inclusion of certain exons, resulting in the production of different protein isoforms. This allows for fine-tuning of gene expression and can have significant effects on cellular functions.

Applications of Recombinant Human ELAVL1 Protein

The unique structure and activity of Recombinant Human ELAVL1 Protein make it a valuable tool for various applications in both basic research and drug development.

One of the most significant applications of this protein is in the study of RNA regulation. By using Recombinant Human ELAVL1 Protein, researchers can investigate the binding specificity and affinity of this protein to different RNA sequences, as well as its effects on RNA stability and alternative splicing. This can provide valuable insights into the mechanisms of gene expression and its dysregulation in diseases such as cancer.

Recombinant Human ELAVL1 Protein has also been used in drug discovery and development. As this protein is involved in cell proliferation and survival, it has been identified as a potential therapeutic target for cancer treatment. By inhibiting its activity, it is possible to decrease the levels of certain proteins that promote tumor growth and survival. Recombinant Human ELAVL1 Protein can also be used in high-throughput screening assays to identify small molecule inhibitors that can target this protein.

Conclusion

In summary, Recombinant Human ELAVL1 Protein is a crucial player in regulating gene expression at the post-transcriptional level. Its unique structure and activity make it an essential tool for studying RNA regulation and its dysregulation in diseases. Additionally, this protein has potential applications in drug discovery and development, particularly in the treatment of cancer. Further research on this protein and its functions may lead to a better understanding of gene expression and the development of new therapies for various diseases.