Recombinant Human EDNRB/ETRB Protein, N-GST & C-His

Description of Recombinant Human EDNRB/ETRB Protein, N-GST & C-His

Introduction to Recombinant Human EDNRB/ETRB Protein

Recombinant Human EDNRB/ETRB Protein, also known as Endothelin receptor type B (ETRB), is a protein that is encoded by the EDNRB gene. It belongs to the G protein-coupled receptor family and is expressed in a variety of tissues, including smooth muscle cells, endothelial cells, and neurons. This protein plays a crucial role in regulating vasoconstriction, cell proliferation, and migration, making it an important target for therapeutic interventions.

Structure of Recombinant Human EDNRB/ETRB Protein

Recombinant Human EDNRB/ETRB Protein is a transmembrane protein, consisting of 442 amino acids with a molecular weight of approximately 50 kDa. It is composed of an extracellular N-terminal domain, seven transmembrane helices, and an intracellular C-terminal domain. The N-terminal domain contains the binding site for the endogenous ligand, endothelin, while the transmembrane helices are responsible for signal transduction. The C-terminal domain is involved in G protein coupling and downstream signaling.

Activity of Recombinant Human EDNRB/ETRB Protein

The main function of Recombinant Human EDNRB/ETRB Protein is to bind to endothelin and activate downstream signaling pathways. Endothelin is a vasoconstrictor peptide that is produced by endothelial cells and plays a crucial role in regulating blood pressure and vascular tone. When endothelin binds to EDNRB, it triggers a cascade of events that leads to vasoconstriction, cell proliferation, and migration. This activity is mediated by G proteins, which are activated upon binding of endothelin to EDNRB.

In addition to its role in vasoconstriction, Recombinant Human EDNRB/ETRB Protein also plays a crucial role in neural crest cell development and migration. Neural crest cells are a group of cells that give rise to various tissues and structures in the body, including the peripheral nervous system, melanocytes, and smooth muscle cells. EDNRB is expressed on the surface of neural crest cells and is required for their migration and differentiation. Mutations in the EDNRB gene have been linked to Hirschsprung’s disease, a disorder characterized by the absence of enteric ganglia in the colon, which is caused by defective neural crest cell migration.

Application of Recombinant Human EDNRB/ETRB Protein

Recombinant Human EDNRB/ETRB Protein has a wide range of applications in both research and therapeutic settings. In research, it is used as an antigen to study the structure and function of the protein. Recombinant EDNRB can be produced in large quantities using recombinant DNA technology, making it a valuable tool for biochemical and biophysical studies.

In therapeutic settings, Recombinant Human EDNRB/ETRB Protein is being investigated as a potential target for the treatment of various cardiovascular diseases, such as hypertension, atherosclerosis, and heart failure. By blocking the activity of EDNRB, it is possible to reduce vasoconstriction and lower blood pressure. In addition, EDNRB antagonists have shown promising results in inhibiting cancer cell proliferation and migration, making them potential candidates for cancer therapy.

Furthermore, Recombinant Human EDNRB/ETRB Protein is also being studied for its potential role in the treatment of Hirschsprung’s disease. By restoring the function of EDNRB, it may be possible to correct the defect in neural crest cell migration and alleviate the symptoms of this disorder.

Conclusion

In summary, Recombinant Human EDNRB/ETRB Protein is a crucial protein involved in regulating vasoconstriction, cell proliferation, and migration. Its structure, activity, and application make it a valuable target for both research and therapeutic purposes. Further studies on this protein may lead to the development of novel treatments for various cardiovascular diseases and disorders related to neural crest cell migration.