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Mouse ENO1 Recombinant Protein

Reference:
size

100ug, 50ug

Brand

ProteoGenix

Product type

Recombinant Proteins

Host Species

Escherichia coli (E. coli)

Applications

Elisa, WB

Product nameMouse ENO1 Recombinant Protein
Uniprot IDP17182
Uniprot linkhttp://www.uniprot.org/uniprot/P17182
Origin speciesHouse Mouse
Expression systemProkaryotic expression
SequenceMGHHHHHHHHHHSSGHIEGRHMMSILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFM GKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLA GNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNIL ENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQ DDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETED TFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLAKDYLDDDDL
Molecular weight50,68 kDa
Protein delivered with Tag?Yes
Purity estimated90%
BufferPBS, pH 7.5
FormFrozen
Delivery conditionDry Ice
Delivery lead time in business days5-7
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandProteoGenix
Host speciesEscherichia coli (E.coli)
Fragment TypeFull-length
Protein AccessionNP_075608
Spec:Entrez GeneID13806
Spec:NCBI Gene AliasesMBP-1, AL022784, 0610008I15, Eno-1
Spec:SwissProtIDP17182
NCBI ReferenceNP_075608
Aliases /SynonymsENO1 Protein, Alpha Enolase Protein, Alpha enolase like 1 Protein, ENO1L1 Protein, Enolase 1 Protein, MBP-1 Protein, MBPB1 Protein, MPB1 Protein, MYC promoter-binding protein 1 Protein, NNE Protein, Phosphopyruvate hydratase Protein, Alpha-enolase Protein, Tau-crystallin Protein, MBP1 Protein, MPB-1 Protein, C-myc promoter-binding protein Protein, Enolase 1, (alpha) Protein, Enolase-alpha Protein, Non-neural enolase Protein, Plasminogen-binding protein Protein, PPH Protein
ReferencePX-P1088
NoteFor research use only

Description of Mouse ENO1 Recombinant Protein

General information on Mouse ENO1 Recombinant Protein:

Enolase 1 (ENO1), commonly known as alpha-enolase is a glycolytic enzyme, one of three enolase isoenzymes found in mammals. Every isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. ENO1 is a glycolytic enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This isozyme is ubiquitously expressed in adult human tissues, including liver, brain, kidney, and spleen. It is a multifunctional enzyme that plays a part in the glycolysis and in various processes such as growth control, hypoxia tolerance and allergic responses. It can also work in the intravascular and pericellular fibrinolytic system because to its capability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons.

Publication

  • 1: Guillou C, Derambure C, Fréret M, Verdet M, Avenel G, Golinski ML, Sabourin_x000D_ JC, Le Loarer F, Adriouch S, Boyer O, Lequerré T, Vittecoq O. Prophylactic_x000D_ Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the_x000D_ Collagen-Induced Arthritis Mice Model. PLoS One. 2015 Aug 24;10(8):e0136359. doi:_x000D_ 10.1371/journal.pone.0136359. eCollection 2015. PubMed PMID: 26302382; PubMed_x000D_ Central PMCID: PMC4547710.
  • _x000D_ _x000D_ _x000D_
  • 2: Sousa LP, Silva BM, Brasil BS, Nogueira SV, Ferreira PC, Kroon EG, Kato K,_x000D_ Bonjardim CA. Plasminogen/plasmin regulates alpha-enolase expression through the _x000D_ MEK/ERK pathway. Biochem Biophys Res Commun. 2005 Dec 2;337(4):1065-71. Epub 2005_x000D_ Oct 4. PubMed PMID: 16225843.
  • _x000D_ _x000D_ _x000D_
  • 3: Lopez-Alemany R, Suelves M, Diaz-Ramos A, Vidal B, Munoz-Canoves P._x000D_ Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 2005 Jan_x000D_ 1;10:30-6. Print 2005 Jan 1. Review. PubMed PMID: 15574344.
  • _x000D_ _x000D_ _x000D_
  • 4: López-Alemany R, Suelves M, Muñoz-Cánoves P. Plasmin generation dependent on_x000D_ alpha-enolase-type plasminogen receptor is required for myogenesis. Thromb_x000D_ Haemost. 2003 Oct;90(4):724-33. PubMed PMID: 14515195.
  • _x000D_ _x000D_ _x000D_
  • 5: Couldrey C, Carlton MB, Ferrier J, Colledge WH, Evans MJ. Disruption of murine_x000D_ alpha-enolase by a retroviral gene trap results in early embryonic lethality. Dev_x000D_ Dyn. 1998 Jun;212(2):284-92. PubMed PMID: 9626503.
  • _x000D_ _x000D_ _x000D_
  • 6: Bottalico LA, Kendrick NC, Keller A, Li Y, Tabas I. Cholesteryl ester loading _x000D_ of mouse peritoneal macrophages is associated with changes in the expression or_x000D_ modification of specific cellular proteins, including increase in an_x000D_ alpha-enolase isoform. Arterioscler Thromb. 1993 Feb;13(2):264-75. PubMed PMID:_x000D_ 8427861.
  • _x000D_ _x000D_ _x000D_
  • 7: Mutze K, Vierkotten S, Milosevic J, Eickelberg O, Königshoff M. Enolase 1_x000D_ (ENO1) and protein disulfide-isomerase associated 3 (PDIA3) regulate_x000D_ Wnt/β-catenin-driven trans-differentiation of murine alveolar epithelial cells._x000D_ Dis Model Mech. 2015 Aug 1;8(8):877-90. doi: 10.1242/dmm.019117. Epub 2015 May_x000D_ 14. PubMed PMID: 26035385; PubMed Central PMCID: PMC4527283.
  • _x000D_ _x000D_ _x000D_
  • 8: Bae S, Kim H, Lee N, Won C, Kim HR, Hwang YI, Song YW, Kang JS, Lee WJ._x000D_ α-Enolase expressed on the surfaces of monocytes and macrophages induces robust_x000D_ synovial inflammation in rheumatoid arthritis. J Immunol. 2012 Jul_x000D_ 1;189(1):365-72. doi: 10.4049/jimmunol.1102073. Epub 2012 May 23. PubMed PMID:_x000D_ 22623332.
  • _x000D_ _x000D_ _x000D_
  • 9: Ryu SY, Hong GU, Kim DY, Ro JY. Enolase 1 and calreticulin regulate the_x000D_ differentiation and function of mouse mast cells. Cell Signal. 2012_x000D_ Jan;24(1):60-70. doi: 10.1016/j.cellsig.2011.07.011. Epub 2011 Jul 23. PubMed_x000D_ PMID: 21803152.
  • _x000D_ _x000D_ _x000D_
  • 10: Wang J, Gu Y, Wang L, Hang X, Gao Y, Wang H, Zhang C. HUPO BPP pilot study: a_x000D_ proteomics analysis of the mouse brain of different developmental stages._x000D_ Proteomics. 2007 Nov;7(21):4008-15. PubMed PMID: 17922513.
  • _x000D_ _x000D_ _x000D_
  • 11: Keller A, Demeurie J, Merkulova T, Géraud G, Cywiner-Golenzer C, Lucas M,_x000D_ Châtelet FP. Fibre-type distribution and subcellular localisation of alpha and_x000D_ beta enolase in mouse striated muscle. Biol Cell. 2000 Oct;92(7):527-35. PubMed_x000D_ PMID: 11229603.
  • _x000D_ _x000D_ _x000D_
  • 12: Merkulova T, Lucas M, Jabet C, Lamandé N, Rouzeau JD, Gros F, Lazar M, Keller_x000D_ A. Biochemical characterization of the mouse muscle-specific enolase:_x000D_ developmental changes in electrophoretic variants and selective binding to other _x000D_ proteins. Biochem J. 1997 May 1;323 ( Pt 3):791-800. PubMed PMID: 9169614; PubMed_x000D_ Central PMCID: PMC1218384.
  • _x000D_ _x000D_ _x000D_
  • 13: Lazar M, Lucas M, Lamandé N, Bishop JG, Gros F, Legault-Demare L. Isolation_x000D_ of murine neuron-specific and non-neuronal enolase cDNA clones. Biochem Biophys_x000D_ Res Commun. 1986 Nov 26;141(1):271-7. PubMed PMID: 3801001.
  • _x000D_ _x000D_ _x000D_
  • 14: Kaghad M, Dumont X, Chalon P, Lelias JM, Lamandé N, Lucas M, Lazar M, Caput_x000D_ D. Nucleotide sequences of cDNAs alpha and gamma enolase mRNAs from mouse brain. _x000D_ Nucleic Acids Res. 1990 Jun 25;18(12):3638. PubMed PMID: 2362815; PubMed Central _x000D_ PMCID: PMC331026.
  • _x000D_ _x000D_ _x000D_
  • 15: Barbieri G, De Angelis L, Feo S, Cossu G, Giallongo A. Differential_x000D_ expression of muscle-specific enolase in embryonic and fetal myogenic cells_x000D_ during mouse development. Differentiation. 1990 Dec;45(3):179-84. PubMed PMID:_x000D_ 2090519.

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