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InVivoMAb Anti-Human IL2 (Iv0020), on SDS-PAGE. The gel was stained overnight with Coomassie Blue. The purity of the antibody is greater than 95%.
Human IL2 recombinant protein(cat. No.PX-P6376) can bind InVivoMAb Anti-Human IL2 (Iv0020) in Western Blot Assay as detected on gel analysis.
Flow-cytometry using anti-human IL2 antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL2 antibody monoclonal antibody (Catalog # ARO-A13359 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PTX17818) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using PE anti-human IL2 antibody. THP-1 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL2 monoclonal antibody (Catalog ARO-A13359, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Human IL2 recombinant protein(cat. No.PX-P6376) at 0.5µg/mL (100µL/well) can bind InVivoMAb Anti-Human IL2 (Iv0020) in indirect ELISA with Goat secondary antibody measured by OD450.
Target Cells were stained with an irrelevant antibody or InVivoMAb Anti-Human IL2 (Iv0020) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
ARO-A13355
ARO-A15737
ARO-A15914
ARO-A15920
ARO-A16073
ARO-A16056
ARO-A10921
PX-P6376
ARO-A11020
ARO-A11022
ARO-A11034
ARO-A11036
PTX19738
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