Human IgG1 (L234A/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC

Human IgG1 (L234A/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC

Biological Function

Human IgG1 (L234A/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC is a monoclonal antibody that specifically binds to the kappa light chain of human immunoglobulin G1 (IgG1). This antibody has been engineered to contain two amino acid mutations (L234A/L235A) that enhance its binding affinity and specificity.

Main Applications

This antibody is commonly used as an isotype control in flow cytometry experiments to distinguish between specific and non-specific binding of antibodies. It can also be used in western blotting and immunohistochemistry applications to ensure the specificity of target antibody binding.

Experimental Use Cases

In flow cytometry experiments, Human IgG1 (L234A/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC is used as a negative control to assess the background fluorescence of cells and to determine the appropriate gating strategy. It is also used to confirm the specificity of target antibody binding by comparing the signal intensity between the isotype control and the target antibody.

In western blotting, this antibody is used as a negative control to ensure that the observed bands are specific to the target protein and not due to non-specific binding. It can also be used in immunohistochemistry to confirm the specificity of target antibody binding and to assess the level of non-specific background staining.

This Human IgG1 (L234A/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC is a highly specific and sensitive tool for flow cytometry, western blotting, and immunohistochemistry experiments. Its enhanced binding affinity and specificity make it an ideal choice for researchers looking to accurately distinguish between specific and non-specific antibody binding.

Structure

To summarize, Human IgG1 (L234A/L235A), kappa Isotype Control Antibody (HyHEL-10), FITC is a monoclonal antibody with two amino acid mutations that enhance its binding affinity and specificity for the kappa light chain of human IgG1. It is commonly used as an isotype control in flow cytometry, western blotting, and immunohistochemistry experiments to ensure the specificity of target antibody binding. This antibody is a valuable tool for researchers looking to accurately assess and control for non-specific binding in their experiments.