Human CD27 HEK293T Stable Cell Line

Human CD27 HEK293T Stable Cell Line: Structure, Activity and Application

Human CD27 HEK293T Stable Cell Line: Structure, Activity and Application

Introduction

The Human CD27 HEK293T Stable Cell Line is a valuable tool for studying the structure, activity and application of CD27, a protein that plays a crucial role in immune response and has been identified as a potential therapeutic target for various diseases. This cell line is derived from human embryonic kidney cells (HEK293T) that have been stably transfected with the gene for CD27. In this article, we will discuss the key features and applications of this cell line.

Structure of Human CD27 HEK293T Stable Cell Line

The Human CD27 HEK293T Stable Cell Line expresses the full-length CD27 protein, which is a member of the tumor necrosis factor receptor superfamily. This protein is composed of four extracellular cysteine-rich domains, a transmembrane domain, and a cytoplasmic tail. The extracellular domains of CD27 are responsible for ligand binding, while the cytoplasmic tail contains signaling motifs that mediate downstream signaling.

Activity of Human CD27 HEK293T Stable Cell Line

The Human CD27 HEK293T Stable Cell Line has been extensively characterized and shown to exhibit similar activity to native CD27-expressing cells. This cell line has been shown to express high levels of CD27 on the cell surface, which allows for efficient binding of its ligand, CD70. Upon ligand binding, CD27 activates downstream signaling pathways, including the NF-κB and MAPK pathways, leading to the production of cytokines and other immune response mediators.

Applications of Human CD27 HEK293T Stable Cell Line

The Human CD27 HEK293T Stable Cell Line has a wide range of applications in both basic research and drug discovery. Here are some of the key applications of this cell line:

1. Studying CD27-mediated immune response

The Human CD27 HEK293T Stable Cell Line can be used to study the role of CD27 in immune response. By manipulating the expression of CD27 or its ligand, CD70, researchers can investigate the impact of this signaling pathway on immune cell activation and function.

2. Screening for CD27-targeted therapeutics

CD27 has been identified as a potential therapeutic target for various diseases, including cancer and autoimmune disorders. The Human CD27 HEK293T Stable Cell Line can be used in high-throughput screening assays to identify compounds that modulate CD27 signaling, which can then be further evaluated for their potential as therapeutics.

3. Flow cytometry analysis

The high expression of CD27 on the surface of the Human CD27 HEK293T Stable Cell Line makes it an ideal tool for flow cytometry analysis. This technique can be used to quantify the levels of CD27 expression, as well as to study the effects of different compounds or treatments on CD27 signaling.

4. Production of recombinant CD27 protein

The Human CD27 HEK293T Stable Cell Line can also be used for the production of recombinant CD27 protein. This protein can be purified and used in various biochemical and structural studies to gain a better understanding of its function and potential as a therapeutic target.

Conclusion

In summary, the Human CD27 HEK293T Stable Cell Line is a valuable tool for studying the structure, activity and applications of CD27. Its high expression of CD27 and similar activity to native cells make it a reliable model for investigating CD27-mediated immune responses and screening for potential therapeutics. With its versatility and ease of use, this cell line is a valuable resource for researchers in the field of immunology and drug discovery.