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Brand: ProteoGenix

FagoFlowEx™ Kit (100 Tests)

Note:
For research use only.

€420.00

+ 420 loyalty points
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FagoFlowEx™ Kit (100 Tests)

FagoFlowEx™ Kit (100 Tests)

Product name FagoFlowEx™ Kit (100 Tests)
Delivery condition Blue ice (+4°C)
Storage condition Store at 2-8°C. Do not freeze.
Brand ProteoGenix
Note For research use only.
Assay type CE IVD
Recovery 80-120%

The FagoFlowEx™ Kit (100 tests) includes the following reagents:

ED7042-1 – E. coli
5 vials containing lyophilized E. coli bacteria.
One vial is intended for stimulation of 20 tubes.

ED7042-2 – DHR123
5 vials containing lyophilized Dihydrorhodamine 123 (DHR123).
One vial is intended for staining of 60 tubes.

ED7042-3 – Stimulation Control
5 vials containing lyophilized PMA (Phorbol 12-myristate 13-acetate).
One vial is intended for stimulation of 20 positive controls.

ED7042-4 – Lysing Solution
15 mL lysing solution.

FagoFlowEx™ Kit for Analysis of Neutrophil Oxidative Burst

The FagoFlowEx™ Kit is designed for the examination of the oxidative (respiratory) burst of neutrophil granulocytes following stimulation with Escherichia coli bacteria in human heparinized whole blood. The assay enables the evaluation of neutrophil activation by measuring the production of reactive oxygen species (ROS) generated during the respiratory burst.

Neutrophils are key components of the innate immune system and contribute to host defense through rapid responses to microbial stimulation. Analysis of the oxidative burst provides valuable information regarding the functional activity of neutrophils in experimental studies involving immune response and host–pathogen interactions.

The FagoFlowEx™ Kit is optimized for flow cytometry analysis, allowing researchers to evaluate neutrophil activation in a controlled and reproducible experimental workflow.

Biological Background

Neutrophil granulocytes play a central role in the innate immune response against invading microorganisms. Upon stimulation by bacterial components such as E. coli, neutrophils undergo a metabolic activation known as the respiratory burst, leading to the production of reactive oxygen species.

These reactive oxygen species contribute to microbial killing and represent an important marker of neutrophil functional activity. Measurement of oxidative burst responses is widely used in research studying:

  • innate immune responses
  • neutrophil activation mechanisms
  • inflammatory processes
  • host–pathogen interactions

Flow cytometry–based assays provide a sensitive method for analyzing these cellular responses in experimental samples.

Applications

The FagoFlowEx™ Kit is suitable for research applications involving:

  • analysis of neutrophil oxidative burst
  • studies of neutrophil activation mechanisms
  • evaluation of innate immune responses
  • research on host–pathogen interactions
  • flow cytometry–based analysis of immune cell function

Researchers studying neutrophil activation and immune responses may also benefit from ProteoGenix expertise in custom antibody production, and recombinant protein production for assay development and validation.

FagoFlowEx™ Kit Price

ProteoGenix offers competitive pricing for flow cytometry assay kits used in immunology research. Each kit provides reagents sufficient for 100 tests, enabling efficient analysis of multiple samples within experimental workflows.

  • Jbeily N, Suckert I, Gonnert FA, Acht B, Bockmeyer CL, Grossmann SD, Blaess MF, Lueth A, Deigner HP, Bauer M, Claus RA: Hyperresponsiveness of mice deficient in plasma-secreted sphingomyelinase reveals its pivotal role in early phase of host response. J Lipid Res. 2013 Feb;54(2):410-24. , URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=jbeily+n+suckert+i+acht+b
  • Jeraiby M, Sidi Yahya K, Depince-Berger AE, Lambert C: Microbicidal activity measured by flow cytometry: Optimization and standardization for detection of primary and functional deficiencies. J Immunol Methods. 2016 Sep 29. pii: S0022-1759(16)30220-4. , URL: https://www.ncbi.nlm.nih.gov/pubmed/27693641
  • Porpodis K, Domvri K, Zarogoulidis P, Petridis D, Tsirgogianni K, Papaioannou A, Hatzizisi O, Kioumis I, Liaka A, Kikidaki V, Lampaki S, Organtzis J, Zarogoulidis K: Roflumilast, a phosphodiesterase-4 inhibitor, induces phagocytic activity in Greek COPD patients. Int J Chron Obstruct Pulmon Dis. 2015 Jun 15;10:1123-8., URL: https://pubmed.ncbi.nlm.nih.gov/26109853/
  • Hromádková L, Zárybnická L, Vávrová J, Bílková Z, Šinkorová Z: The impact of pre-analytical treatment and sorting on human neutrophil function. Veterinární Medicína, 59, 2014 (11): 543-552 , URL: https://www.researchgate.net/publication/279320205_The_impact_of_pre-analytical_treatment_and_sorting_on_human_neutrophil_function
  • Miková E, Černý V, Novotná O, Petrásková P, Boráková K, Hel Z, Hrdý J: Immature neutrophils in cord blood exert increased expression of genes associated with antimicrobial function. Front Immunol. 2024 Mar 26:15:1368624., URL: https://pubmed.ncbi.nlm.nih.gov/38596677/

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