The FagoFlowEx Kit is intended for the examination of phagocytic activity of neutrophil granulocytes by measuring the respiratory (oxidative) burst after their stimulation with E. coli bacteria in human heparinized whole blood using flow cytometry.
Kit content:
ED7042-1 E.coli – 5 vials containing lyophilized E. coli bacteria, 1 vial is intended for stimulation of 20 tubes.
ED7042-2 DHR123 – 5 vials containing lyophilized Dihydrorhodamine 123, 1 vial is intended for staining of 60 tubes.
ED7042-3 Stimulation Control – 5 vials containing lyophilized PMA (Phorbol 12-myristate 13-acetate), 1 vial is intended for stimulation of 20 positive controls.
ED7042-4 Lysing Solution – 15 ml
Publication
Jbeily N, Suckert I, Gonnert FA, Acht B, Bockmeyer CL, Grossmann SD, Blaess MF, Lueth A, Deigner HP, Bauer M, Claus RA: Hyperresponsiveness of mice deficient in plasma-secreted sphingomyelinase reveals its pivotal role in early phase of host response. J Lipid Res. 2013 Feb;54(2):410-24. , URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=jbeily+n+suckert+i+acht+b
Jeraiby M, Sidi Yahya K, Depince-Berger AE, Lambert C: Microbicidal activity measured by flow cytometry: Optimization and standardization for detection of primary and functional deficiencies. J Immunol Methods. 2016 Sep 29. pii: S0022-1759(16)30220-4. , URL: https://www.ncbi.nlm.nih.gov/pubmed/27693641
Porpodis K, Domvri K, Zarogoulidis P, Petridis D, Tsirgogianni K, Papaioannou A, Hatzizisi O, Kioumis I, Liaka A, Kikidaki V, Lampaki S, Organtzis J, Zarogoulidis K: Roflumilast, a phosphodiesterase-4 inhibitor, induces phagocytic activity in Greek COPD patients. Int J Chron Obstruct Pulmon Dis. 2015 Jun 15;10:1123-8., URL: https://pubmed.ncbi.nlm.nih.gov/26109853/
Miková E, Černý V, Novotná O, Petrásková P, Boráková K, Hel Z, Hrdý J: Immature neutrophils in cord blood exert increased expression of genes associated with antimicrobial function. Front Immunol. 2024 Mar 26:15:1368624., URL: https://pubmed.ncbi.nlm.nih.gov/38596677/
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