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Envafolimab Biosimilar - Anti-CD274;PD-L1 mAb - Research Grade can bind to its target in indirect ELISA with Goat secondary antibody measured by OD450.
Target Cells were stained with an irrelevant antibody or Envafolimab Biosimilar - Anti-CD274;PD-L1 mAb - Research Grade at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
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