4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
Brand
ProteoGenix
Host species
Escherichia coli (E.coli)
Fragment Type
Partial
NCBI Reference
NP_588151.1
Aliases /Synonyms
Peptide -N-Glycosidase F, PNGase F, PNGaseF
Reference
PX-P3070
Note
For research use only
Description of Elizabethkingia miricola PNGaseF Recombinant Protein
General information on Elizabethkingia miricola PNGaseF Recombinant Protein-15000u
PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli. It cleaves a complete glycan from a glycoprotein and it deaminates the asparagine to aspartic acid, but leaves the oligosaccharide undamaged. PNGase F will not eliminate oligosaccharides containing Alpha-(1, 3)-linked core fucose usually found in plant glycoproteins. A tri-peptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F.
1: Kuhn P, Tarentino AL, Plummer TH Jr, Van Roey P. Crystal structure of
peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F at 2.2-A
resolution. Biochemistry. 1994 Oct 4;33(39):11699-706. PubMed PMID: 7918386.
2: Norris GE, Stillman TJ, Anderson BF, Baker EN. The three-dimensional structure
of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum.
Structure. 1994 Nov 15;2(11):1049-59. PubMed PMID: 7881905.
3: Kuhn P, Guan C, Cui T, Tarentino AL, Plummer TH Jr, Van Roey P. Active site
and oligosaccharide recognition residues of
peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F. J Biol Chem. 1995
Dec 8;270(49):29493-7. PubMed PMID: 7493989.
4: Lemp D, Haselbeck A, Klebl F. Molecular cloning and heterologous expression of
N-glycosidase F from Flavobacterium meningosepticum. J Biol Chem. 1990 Sep
15;265(26):15606-10. PubMed PMID: 2203781.
5: Barsomian GD, Johnson TL, Borowski M, Denman J, Ollington JF, Hirani S,
McNeilly DS, Rasmussen JR. Cloning and expression of
peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F in Escherichia
coli. J Biol Chem. 1990 Apr 25;265(12):6967-72. PubMed PMID: 2182635.
6: Tarentino AL, Quinones G, Trumble A, Changchien LM, Duceman B, Maley F,
Plummer TH Jr. Molecular cloning and amino acid sequence of
peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase from flavobacterium
meningosepticum. J Biol Chem. 1990 Apr 25;265(12):6961-6. Erratum in: J Biol Chem
1990 Jul 5;265(19):11405. PubMed PMID: 2182634.
Waltteri Hosia –
Was the protein active?: Yes
Very good product. I would buy again.
Waltteri Hosia –
Was the protein active?: Yes
Very good product. I would buy again.
Waltteri Hosia –
Was the protein active?: Yes
Very good product. I would buy again.