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Stromal cell-derived factor 1(CXCL12)(cat. No.PX-P4857) at 0.5µg/mL (100µL/well) can bind Anti-Human CXCL12/SDF-1 Antibody (SAA0467) in indirect ELISA with Goat secondary antibody measured by OD450.
Flow-cytometry using anti-human CXCL12 antibody.MDA-MB-453 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CXCL12 antibody monoclonal antibody (Catalog # ARO-A15487 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PTX17818) and cells analysed on a NovoCyte Flow Cytometer.
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