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Stromal cell-derived factor 1(CXCL12)(cat. No.PX-P4857) at 0.5µg/mL (100µL/well) can bind Anti-Human CXCL12/SDF-1 Antibody (SAA0467) in indirect ELISA with Goat secondary antibody measured by OD450.
Target Cells were stained with an irrelevant antibody or Anti-Human CXCL12/SDF-1 Antibody (SAA0467) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG Polyclonal Antibody, FITC (PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
PTX18549
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