Monoclonal antibody production package details
- Antigen design (design of the most appropriate fragment to be produced)
- Two gene syntheses (each gene optimized for a specific expression system) and two subclonings
- Expression tests in two systems taken from E. coli, B. subtilis, P. pastoris, insect cells, and mammalian cells. To be determined by ProteoGenix. GST-tagged version also done in parallel for IP experiments
- Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
- Mice injections (5 mice)
- Immune response control by IP
- Selection of the best two mice for two fusions in order to have a large number and variety of clones
- Screening of the fusion product by IP
- Subcloning of two parental clones (2-3 rounds of limiting dilutions)
- Production and purification of 2mg from the best clone
- Level 1 : If we don’t succeed in producing an antibody that detects the immunogen by IP, we charge EUR 1 000
- Level 2 : If we succeed in producing an antibody that works using IP against the immunogen, we charge a part of the cost
- Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
- 10 -20 best clones at parental stage for customer’s selection
- 100 µg of purified antibody to confirm hybridoma purchase
- 50 µg of purified protein
- 1.9 mg of purified antibody
- One hybridoma cell line (four frozen vials)
- 150 µg of purified protein
- Gene in pUC57
- Gene synthesis: 3-4 weeks
- Protein production : 7-9 weeks
- Injections: 8-12 weeks
- Fusion & screening:5-6 weeks
- Subcloning: 8-12 weeks
- Antibody production:3-4 weeks
- Total: 34-47 weeks
Our application guaranteed monoclonal antibody production packages
Standard ELISA: ATX-PACK1M
Premium ELISA: ATX-PACK2M
Premium WB: ATX-PACK3M
Premium Flow Cytometry: ATX-PACK4M
Premium sandwich ELISA: ATX-PACK5M
Premium IF: ATX-PACK6M
Peptide: ATX-PACK8M
Modification specific: ATX-PACK9M
Premium IHC: ATX-PACK10M