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Gene synthesis services

Custom gene synthesis services

Gene synthesis from €0.14/bp · Sanger-verified

Order custom gene synthesis online: enter your DNA sequence, select your cloning options and get an instant quote.

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No Gene, No Fee

Pay only when synthesis succeeds.

Configure & Get Instant Price → Request a Quote →

Have specific requirements or prefer direct contact? Our team can help.

  • 5 days
    To obtain your gene

  • 150+
    Vector options

  • AI-powered
    Codon optimization

  • Sanger
    Sequence accuracy guaranteed

Get your instant
gene synthesis quote

Use our online ordering platform to submit your DNA sequence and configure your project in just a few steps.
Complete the form below to obtain an instant gene synthesis price estimate and start your project.

 

GENE SYNTHESIS PRICING

Clear Pricing for Gene Synthesis & Cloning

Gene synthesis starts at €0.14/bp, with cloning options available to match your project requirements.

Gene Synthesis Pricing

Gene length Price Turnaround
<400 bp €60/unit 5 business days
400–3,000 bp €0.14/bp 5 business days
3,001–4,000 bp €0.20/bp 10 business days
4,001–6,000 bp €0.22/bp 10 business days
>6,000 bp On request Request a quote

Subcloning

Option Price
Amplification vector €25/unit
Expression vector €25/unit
Customer’s vector €50/unit

FROM SEQUENCE TO VERIFIED GENE

Gene Synthesis Workflow

A connected three-step process from sequence preparation to synthesis, cloning and verification.

STEP 01

 
 
 
 
 

Sequence Preparation & Codon Optimization

Prepare your sequence for synthesis, with optional codon optimization tailored to your selected expression host.

STEP 02

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DNA Synthesis & Assembly

Gene synthesis and assembly, with options for tags, signal peptides, mutations, truncations and domain swaps.

STEP 03

 
 
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Cloning & Sequence Verification

Cloning into the selected vector, followed by Sanger sequence verification and quality-control documentation.

GENE SYNTHESIS DELIVERABLES

What’s Included in Your Gene Synthesis Order?

Your order includes the synthesized gene, sequence verification and quality-control documentation. Final specifications depend on your selected options.

  • 01

    Your Synthesized Gene

    For a standard research-grade cloned gene, the default quantity is 4 µg. Quantity and preparation grade can be customized.

  • 02

    Sequence Verification

    Your gene is checked by Sanger sequencing before delivery.

  • 03

    Certificate of Analysis (CoA)

    Quality-control documentation accompanies the gene synthesis service.

AI-BASED SEQUENCE OPTIMIZATION

Why use AI-Powered codon optimization?

Need to adapt your gene for a specific expression host? Run sequence analysis and review the optimized result immediately, without leaving the ordering workflow.

Optimize your sequence for expression

  1. Support expression in your chosen host Adapt codon usage to the target expression system.

  2. Reduce potential translation bottlenecks Identify and address rare-codon patterns that may affect translation.
  3. Address sequence-related constraints Consider GC content, repeats and other sequence features during optimization.

CLONING OPTIONS

Choose the Right Vector for Your Gene

Choose from 150+ vector options across multiple expression systems to match your gene synthesis project requirements.

SUPPORTED EXPRESSION SYSTEMS
Mammalian Bacterial Yeast Insect / Baculovirus Plant Lentiviral

Trust experts in complex gene synthesis

When automated platforms reach their limits, our gene synthesis platform steps in. We routinely synthesize and deliver complex constructs.

Complexity feature
ProteoGenix capabilities
Direct repeats
Direct repeats longer than 400 bp, separated by less than 100 bp
Reverse repeats / hairpins
Inverted repeats longer than 20 bp, separated by less than 40 bp
Short tandem repeats
Tandem repeat regions exceeding 1,200 bp
Extreme GC content
Sequences with very low or very high GC content, including regions approaching 0% or 100%
Homopolymers / Poly-A
Poly-T stretches longer than 20 bp or Poly-A stretches longer than 100 bp

Working with a difficult DNA sequence? Discuss your sequence characteristics with our scientists to assess feasibility.

Discuss Your Project →

EXPERIMENTAL EVIDENCE

Proven Results on Challenging Gene Synthesis

Two examples of complex DNA constructs successfully delivered by ProteoGenix, supported by project-specific experimental data.

CASE STUDY 01 · HIGH-GC GENE

72% GC

High-GC Gene Synthesis

2.4 kb gene · Localized regions above 85% GC

GC content profile of a high-GC gene synthesis project, showing local GC variation along the sequence

GC content profile provided with the project documentation.

PROJECT CHALLENGE

Dense hairpins and G-quadruplexes interfering with standard automated assembly.

REPORTED RESULT

Sanger-verified sequence accuracy with no frameshifts, delivered in a stable plasmid vector.

CASE STUDY 02 · POLY-A CONSTRUCT

30 A · 10 bp · 74 A

Interrupted Poly-A Construct

Two poly-A segments separated by a 10 bp interruption

Forward and reverse Sanger sequencing chromatograms for an interrupted poly-A construct

Forward and reverse Sanger sequencing traces supplied for the construct.

PROJECT CHALLENGE

Polymerase slippage, recombination-mediated deletions and Sanger trace collapse.

REPORTED RESULT

Delivered without sequence truncation using specialized host strains and custom assembly protocols.

WHY PROTEOGENIX

More Than Gene Synthesis. A Scientific Partner for Your Research.

The right DNA construct starts with the right experimental strategy. At ProteoGenix, we consider how your gene will be used to help guide decisions on sequence optimization, vector selection and cloning.

DESIGNED AROUND YOUR RESEARCH

Protein expression, functional studies and cell line development can call for different construct requirements. Our scientific team helps you evaluate the options before synthesis begins.

  1. Start with the application Consider the intended experimental use of your DNA construct.
  2. Review construct choices Discuss codon optimization, vector options and cloning requirements.
  3. Move forward with clarity Align the synthesis approach with your research objectives before production.

Trusted by our clients

The optimized genes ordered were of excellent quality, were cost-effective and perfectly adapted for biochemical and biophysical studies. In addition, the account manager in charge of the service advised us really well before and after the sales process, and we got the constructs on time. 

Julia Asencio Hernández, Project Leader, CASC4DE

casc4de with gene synthesis company

Our expertise demonstrated through scientific publications

ProteoGenix gene synthesis services cited in recent publications across leading journals.

Publications

Moss BRCA2 lacking the canonical DNA-binding domain promotes homologous recombination and binds to DNA.

Alice Chanteau, Suliane Quilleré, Arthur Crouset, Sreejith Allipra, Ulysse Tuquoi, Pierre-François Perroud, Simona Miron, Pauline Dupaigne, Sophie Zinn-Justin, Fabien Nogué, Rajeev Kumar. Nucleic Acids Research. 2025;53(17):gkaf856.

 

 

Publications

First evidence of efficacy of peptides targeting the pUL56-pUL89 interaction domain of the human cytomegalovirus terminase complex.

Sarah Mafi, Jean-Luc Poyet, Sophie Alain, Gaëtan Ligat, Sébastien Hantz. Antiviral Research. 2025;242:106259.

Publications

An Engineered SARS-CoV-2 S1 Glycoprotein Produced in Pichia pastoris as a Candidate Vaccine Antigen.

Sanaz Majidi, Khosrow Aghaiypour Kolyani, Meisam Akrami, Maryam Dadar. Molecular Biotechnology, published in 2025, vol 68 in 2026.

Publications

Cecropin anisaxin-2S has in vitro immunomodulatory, but not antiproliferative and antiviral properties.

Jovana Majstorović, Anush Arakelyan, Željka Trumbić, Jan Kamiš, Mikolaj Adamek, Tomislav Rončević, et al. Frontiers in Immunology. 2025;16:1567505.

Custom gene synthesis — frequently asked questions

LET’S DISCUSS YOUR PROJECT

Need a Custom Gene Synthesis Quote?

Have specific requirements or prefer to discuss your project directly? Send us your details and our team will help you find the right solution.

  • Custom sequences and cloning requirements
  • Complex gene synthesis projects
  • Direct assistance from our team

? Already have your project details?

Share your sequence or requirements directly with our team to request a quote.

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