Can you work with any antibody format?

Yes. Our antibody humanization service is fully adaptable and can be applied to all antibody formats, including IgG, VHH (nanobodies), bispecific antibodies, and other engineered constructs. We tailor the humanization strategy to your molecule’s structure, species origin, and project goals.

What does your humanization workflow look like from sequence analysis to final validation?

We start by sequencing the parental antibody (hybridoma-derived) and confirming the sequences through analysis. We then express and purify a chimeric version with full QC (SDS-PAGE, WB, UV280) before designing humanized variants using bioinformatics, germline selection, structural modeling, and in silico CDR grafting. Next, we produce at least 18 recombinant humanized variants and validate them through affinity and developability testing, including ELISA, SEC-HPLC (aggregation), and SPR (Biacore/Horiba).

How long does a full humanization campaign typically take?

A full antibody humanization campaign typically takes 10–15 weeks, depending on the number of variants produced and the extent of functional validation required.Yes. We provide multiple humanized antibody variants (at least 18 purified samples), allowing you to compare candidates and select the best option based on affinity, specificity, and developability.

What data packages do you deliver at the end of the project?

At the end of the project, we deliver a complete data package including purified humanized antibody variants, the full antibody sequences, and detailed analytical. We provide full recombinant expression, purification, and characterization of the humanized variants, including QC and functional validation assays such as ELISA, SEC-HPLC, and SPR.

What in vitro tests do you use to confirm that binding properties are maintained?

To confirm that binding properties are maintained, we perform in vitro affinity and kinetics testing using SPR (surface plasmon resonance), providing precise measurements of antibody–antigen interactions.

Can you guarantee functional retention after humanization (affinity, specificity, developability)?

While functional retention cannot be absolutely guaranteed for every antibody, we strongly secure your project through a no win/no fee commitment and a go/no-go decision points. This allows you to evaluate early functional results before moving forward with full humanization and variant production.

Do you provide multiple humanized variants so we can select the best candidate?

Yes. We provide multiple humanized antibody variants (at least 18 purified samples), allowing you to compare candidates and select the best option based on affinity, specificity, and developability.

What is included in your pricing — sequence analysis only, or full expression and validation?

Our pricing is flexible and depends on your project needs. We offer both full humanization packages (including sequence analysis, variant design, recombinant expression, purification, and validation) and customized options, allowing you to select only the services you require.

How easily can we transition from humanization to further services such as affinity maturation or stable cell line development?

Transitioning is straightforward. From antibody engineering to large-scale production, our services work in full synergy, enabling seamless progression from humanization to affinity maturation, developability, or stable cell line development at any stage of your project.

Who owns the IP of the humanized sequences?

You retain 100% ownership of the IP. All humanized antibody sequences and project results are delivered fully IP-free, with complete freedom for your future development and commercialization.

Antibody Humanization Service

Engineer your antibody to maximise your chances to go to the clinic

Purified antibody samples

IP free

Suitable for all antibody formats and species

Humanize your antibody thanks to our high-quality expertise

We experimentally test some variants to identify the optimal solution for your antibody. You benefits at least 18 purified antibody samples with sequences ready for the next stage of your project.

Receive at least 18 humanized antibody variants

We provide high-quality expertise to humanize your antibody with rigor and a structured approach, maximizing your chances of advancing to the clinic. We experimentally test multiple variants to confirm the best candidates before moving on to the next development steps. You receive at least 18 purified ready-to-use humanized antibody variants, prepared for the next stage of your project. We also deliver a detailed report including the antibody sequences to make your in-house work easier.

Our “no win, no fee” guarantee ensures you only pay if we deliver the expected results.

Parental antibody sequencing from hybridoma

Chimeric antibody expression and purification

Design and production of 18 humanized antibody variants

Characterization of humanized monoclonal antibody variants

Humanized your antibody without compromising the biological functions

Besides humanization, we take into account several critical parameters to derisk your antibody as much as possible. You save time and money by increasing your chances of success and accelerating your antibody’s access to the clinic.

The immunogenicity of your antibody is reduced while preserving its biological functions thanks to a simplified process combined with high-productivity expression systems.

We adapt to all your antibody formats and species

Our antibody humanization service is fully customized, even for complex projects: it adapts to all antibody formats and species.

With dedicated end-to-end support from PhD-level experts, we can meet every project requirement by designing and evaluating multiple options to identify the most suitable solution for your antibody. From antibody engineering to large-scale production, our services work in full synergy, enabling seamless management of every stage of your project.

Go/No Go checkpoints throughout the process allow you to quickly decide whether to continue or adjust the strategy according to your needs.

To ensure you have complete freedom, all results are 100% yours—no intellectual property restrictions, you retain complete control over your antibodies.

Optimize Wet-Lab with proprietary AI

Thanks to a fully transparent process powered by artificial intelligence, you can optimize your antibody while simplifying your operations and accelerating your results.

Get your antibody with AI

FAQ

Yes. Our antibody humanization service is fully adaptable and can be applied to all antibody formats, including IgG, VHH (nanobodies), bispecific antibodies, and other engineered constructs. We tailor the humanization strategy to your molecule’s structure, species origin, and project goals.
We start by sequencing the parental antibody (hybridoma-derived) and confirming the sequences through analysis. We then express and purify a chimeric version with full QC (SDS-PAGE, WB, UV280) before designing humanized variants using bioinformatics, germline selection, structural modeling, and in silico CDR grafting. Next, we produce at least 18 recombinant humanized variants and validate them through affinity and developability testing, including ELISA, SEC-HPLC (aggregation), and SPR (Biacore/Horiba).
A full antibody humanization campaign typically takes 10–15 weeks, depending on the number of variants produced and the extent of functional validation required.Yes. We provide multiple humanized antibody variants (at least 18 purified samples), allowing you to compare candidates and select the best option based on affinity, specificity, and developability.
At the end of the project, we deliver a complete data package including purified humanized antibody variants, the full antibody sequences, and detailed analytical. We provide full recombinant expression, purification, and characterization of the humanized variants, including QC and functional validation assays such as ELISA, SEC-HPLC, and SPR.
While functional retention cannot be absolutely guaranteed for every antibody, we strongly secure your project through a no win/no fee commitment and a go/no-go decision points. This allows you to evaluate early functional results before moving forward with full humanization and variant production.
Our pricing is flexible and depends on your project needs. We offer both full humanization packages (including sequence analysis, variant design, recombinant expression, purification, and validation) and customized options, allowing you to select only the services you require.
Transitioning is straightforward. From antibody engineering to large-scale production, our services work in full synergy, enabling seamless progression from humanization to affinity maturation, developability, or stable cell line development at any stage of your project.

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Antibody Humanization Service

Engineer your antibody to maximise your chances to go to the clinic

Humanize your antibody thanks to our high-quality expertise

Receive at least 18 humanized antibody variants

Humanized your antibody without compromising the biological functions

We adapt to all your antibody formats and species

100% IP Free : Full IP Free ownership, it is your antibody

Optimize Wet-Lab with proprietary AI

FAQ

Need a quote or more information? Contact us!

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Antibody Humanization Service

Engineer your antibody to maximise your chances to go to the clinic

Humanize your antibody thanks to our high-quality expertise

Receive at least 18 humanized and characterized antibody variants

Your antibody delivered with functional validation

With multiple guarantees for your antibody

Receive at least 18 humanized antibody variants

Humanized your antibody without compromising the biological functions

We adapt to all your antibody formats and species

Optimize Wet-Lab with proprietary AI

FAQ

Can you work with any antibody format?

What does your humanization workflow look like from sequence analysis to final validation?

How long does a full humanization campaign typically take?

What data packages do you deliver at the end of the project?

What in vitro tests do you use to confirm that binding properties are maintained?

Can you guarantee functional retention after humanization (affinity, specificity, developability)?

Do you provide multiple humanized variants so we can select the best candidate?

What is included in your pricing — sequence analysis only, or full expression and validation?

How easily can we transition from humanization to further services such as affinity maturation or stable cell line development?

Who owns the IP of the humanized sequences?

Need a quote or more information? Contact us!

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