Recombinant Human TOP2B Protein, N-His

Description of Recombinant Human TOP2B Protein, N-His

Title: Introduction to Recombinant Human TOP2B Protein

Recombinant Human TOP2B Protein, also known as DNA topoisomerase II beta, is a key enzyme involved in DNA replication and transcription. This protein is encoded by the TOP2B gene and is a member of the topoisomerase family, which plays a crucial role in maintaining the integrity and stability of the genome. In this article, we will explore the structure, activity, and applications of Recombinant Human TOP2B Protein.

Title: Structure of Recombinant Human TOP2B Protein

Recombinant Human TOP2B Protein is a 170 kDa protein that consists of 1531 amino acids. It is composed of four functional domains: an N-terminal domain, a central core domain, a C-terminal domain, and a C-terminal acidic tail. The N-terminal domain contains the DNA binding region, while the central core domain is responsible for the catalytic activity of the enzyme. The C-terminal domain is involved in protein-protein interactions, and the C-terminal acidic tail is essential for the regulation of enzyme activity. The structure of Recombinant Human TOP2B Protein is highly conserved among different species, indicating its critical role in cellular processes.

Title: Activity of Recombinant Human TOP2B Protein

Recombinant Human TOP2B Protein is a type II topoisomerase, which means it is involved in the breaking and rejoining of double-stranded DNA. This enzyme is essential for DNA replication, transcription, and chromosome segregation. It works by creating a transient double-strand break in the DNA, allowing it to unwind and relieve the tension caused by the supercoiling of the DNA. This activity is crucial for the proper functioning and maintenance of the genome. Mutations in the TOP2B gene have been linked to various diseases, including cancer and developmental disorders, highlighting the importance of this protein in cellular processes.

Title: Applications of Recombinant Human TOP2B Protein

Recombinant Human TOP2B Protein has numerous applications in both research and therapeutic settings. It is widely used in the study of DNA replication, transcription, and chromosome dynamics. This protein has also been utilized in drug discovery and development, as it is a target for several anti-cancer drugs, such as etoposide and doxorubicin. These drugs work by inhibiting the activity of TOP2B, leading to the death of cancer cells. Recombinant Human TOP2B Protein is also used in the production of vaccines, as it can be used as an antigen to elicit an immune response against specific diseases.

Title: Production of Recombinant Human TOP2B Protein

Recombinant Human TOP2B Protein is produced using recombinant DNA technology, where the TOP2B gene is inserted into a suitable expression vector and then expressed in a host cell, such as E. coli or insect cells. The protein is then purified using various chromatography techniques to obtain a highly pure and active form. The availability of recombinant TOP2B Protein has greatly facilitated its use in research and therapeutic applications.

Title: Conclusion

In conclusion, Recombinant Human TOP2B Protein is a crucial enzyme involved in DNA replication and transcription. Its structure and activity make it an essential player in maintaining the integrity and stability of the genome. This protein has various applications in research and therapeutics, and its production using recombinant DNA technology has greatly enhanced its availability and use. Further research on this protein may lead to a better understanding of its role in disease processes and potential new therapeutic targets.