Recombinant Human TMPRSS4, N-His

Description of Recombinant Human TMPRSS4, N-His

Recombinant Human TMPRSS4: Structure, Activity, and Applications

Introduction

Recombinant proteins have become essential tools in the field of biotechnology, offering a wide range of applications in research, diagnostics, and therapeutics. One such protein is the human Transmembrane Protease Serine 4 (TMPRSS4), which has gained significant attention due to its role in cancer progression and metastasis. In this article, we will discuss the structure, activity, and applications of recombinant human TMPRSS4.

Structure of Recombinant Human TMPRSS4

TMPRSS4 is a type II transmembrane serine protease, consisting of 492 amino acids. It belongs to the family of serine proteases, which are enzymes that cleave other proteins at specific sites. The protein is composed of four domains: a cytoplasmic domain, a transmembrane domain, a stem region, and a catalytic domain. The catalytic domain is responsible for the proteolytic activity of TMPRSS4, while the stem region and transmembrane domain anchor the protein to the cell membrane.

Recombinant Protein Production

Recombinant human TMPRSS4 is produced through genetic engineering techniques, where the gene for TMPRSS4 is inserted into a suitable expression vector and transferred into host cells, such as bacteria, yeast, or mammalian cells. The protein is then produced in large quantities using fermentation or cell culture methods.

Activity of Recombinant Human TMPRSS4

TMPRSS4 plays a crucial role in cancer progression and metastasis by promoting the activation of other proteins involved in these processes. It has been shown to activate the hepatocyte growth factor (HGF) and its receptor c-Met, which are essential for cancer cell migration and invasion. TMPRSS4 also activates the epidermal growth factor receptor (EGFR), which is involved in cell proliferation and survival. Additionally, TMPRSS4 has been found to cleave and activate the protease-activated receptor 2 (PAR2), which is associated with inflammation and tumor growth.

Applications of Recombinant Human TMPRSS4

Recombinant human TMPRSS4 has a wide range of applications in both research and clinical settings. Here are some of the key applications of this protein:

Antigen for Antibody Production

Recombinant human TMPRSS4 can be used as an antigen to generate antibodies for research purposes. These antibodies can be used to study the expression and localization of TMPRSS4 in different tissues and cell types, as well as its role in cancer progression and metastasis.

Diagnostics

TMPRSS4 has been identified as a potential biomarker for various types of cancer, including lung, breast, and pancreatic cancer. Recombinant human TMPRSS4 can be used to develop diagnostic tests for the early detection and monitoring of these cancers.

Therapeutics

Given its role in cancer progression and metastasis, TMPRSS4 has emerged as a potential target for cancer therapy. Recombinant human TMPRSS4 can be used to screen for small molecule inhibitors that can block its activity and potentially inhibit cancer growth and metastasis.

Potential for Other Applications

Apart from its role in cancer, TMPRSS4 has also been implicated in other diseases, such as respiratory infections and inflammatory bowel disease. Recombinant human TMPRSS4 can be used to further study the role of this protein in these conditions and potentially develop new treatments.

Conclusion

Recombinant human TMPRSS4 is a crucial protein with diverse functions and applications. Its structure, activity, and potential for various applications make it a promising target for further research and development. With ongoing advancements in biotechnology, we can expect to see more applications of this protein in the future, leading