Recombinant Human NUMA1/NMP-22 Protein, N-His

Description of Recombinant Human NUMA1/NMP-22 Protein, N-His

Introduction

Recombinant Human NUMA1/NMP-22 Protein is a highly specific and sensitive antigen used in various scientific and medical applications. It is a recombinant protein produced through genetic engineering techniques and has a wide range of uses due to its unique structure and activity.

Structure of Recombinant Human NUMA1/NMP-22 Protein

The recombinant protein is a fusion protein consisting of two domains – the N-terminal domain of human NUMA1 (nuclear mitotic apparatus protein 1) and the C-terminal domain of human NMP-22 (nuclear matrix protein 22). The two domains are joined by a flexible linker peptide, resulting in a single protein with a molecular weight of approximately 95 kDa.

The N-terminal domain of NUMA1 is responsible for the protein’s localization to the mitotic spindle during cell division, while the C-terminal domain of NMP-22 is involved in DNA binding and chromatin organization. The fusion of these two domains in the recombinant protein allows for its specific binding to certain cellular structures and molecules.

Activity of Recombinant Human NUMA1/NMP-22 Protein

The recombinant protein has multiple activities, making it a valuable tool in various research and diagnostic applications. Its primary function is as an antigen, specifically recognized by antibodies in the serum of patients with bladder cancer. This makes it a useful biomarker for the early detection of bladder cancer.

In addition to its role as an antigen, the recombinant protein also has DNA-binding activity, similar to the native NMP-22 protein. This allows for its use in chromatin immunoprecipitation (ChIP) assays, where it can be used to identify DNA sequences that are bound by specific proteins. It can also be used in protein-protein interaction studies, as it has been shown to interact with other nuclear proteins involved in cell division and DNA repair.

Applications of Recombinant Human NUMA1/NMP-22 Protein

The recombinant protein has a wide range of applications in both research and clinical settings. Its most important application is in the early detection of bladder cancer. It is used in the NMP-22 BladderChek test, a non-invasive urine test that detects the presence of NMP-22 protein in the urine. This test is highly sensitive and specific, making it a valuable tool for the early detection of bladder cancer.

Aside from bladder cancer detection, the recombinant protein is also used in research studies to investigate its role in cell division and DNA repair. Its DNA-binding activity allows for the identification of specific DNA sequences and its protein-protein interaction capabilities make it a useful tool in studying protein complexes involved in these processes.

Furthermore, the recombinant protein has potential applications in drug discovery and development. Its unique structure and activity make it a potential target for drug development, particularly in the treatment of bladder cancer and other diseases involving cell division and DNA repair.

Conclusion

In summary, Recombinant Human NUMA1/NMP-22 Protein is a valuable tool in scientific and medical research, with its specific structure, activity, and applications. Its use as an antigen in the early detection of bladder cancer, as well as its potential in drug development and research studies, make it an important protein in the field of biology and medicine.