Recombinant Human ATG14 Protein, N-His

Description of Recombinant Human ATG14 Protein, N-His

Title: Introduction to Recombinant Human ATG14 Protein

Recombinant Human ATG14 protein, also known as Autophagy Related Protein 14, is a key component of the autophagy machinery. Autophagy is a cellular process that involves the degradation and recycling of damaged or unnecessary cellular components. This process is crucial for maintaining cellular homeostasis and is involved in various physiological and pathological conditions. Recombinant Human ATG14 protein plays a critical role in regulating autophagy and has been extensively studied for its structure, activity, and potential applications.

Title: Structure of Recombinant Human ATG14 Protein

Recombinant Human ATG14 protein is a 46 kDa protein consisting of 408 amino acids. It contains a C-terminal WD40 domain, which is essential for its interaction with other proteins involved in autophagy, such as Beclin-1 and Vps34. The N-terminal region of ATG14 contains a coiled-coil domain, which is responsible for its dimerization and interaction with other autophagy-related proteins. The crystal structure of recombinant Human ATG14 protein has been determined, revealing its unique structural features and providing insights into its function.

Title: Activity of Recombinant Human ATG14 Protein

Recombinant Human ATG14 protein is a key regulator of autophagy initiation. It forms a complex with Beclin-1 and Vps34, which is essential for the formation of the pre-autophagosomal structure (PAS) and the recruitment of other autophagy-related proteins. This complex acts as a lipid kinase, phosphorylating phosphatidylinositol to generate phosphatidylinositol 3-phosphate (PI3P). PI3P is crucial for the recruitment of other autophagy-related proteins, such as WIPI2 and ATG16L1, to the PAS and the subsequent formation of the autophagosome. Recombinant Human ATG14 protein also plays a role in regulating the size and maturation of autophagosomes, as well as the fusion of autophagosomes with lysosomes for degradation.

Title: Applications of Recombinant Human ATG14 Protein

Due to its crucial role in regulating autophagy, recombinant Human ATG14 protein has potential applications in various fields. One of the major applications is in cancer research, as autophagy has been shown to play a role in cancer development and progression. Studies have shown that recombinant Human ATG14 protein can inhibit tumor growth by promoting autophagy-mediated cell death. It has also been implicated in neurodegenerative diseases, as autophagy dysfunction is a common feature in these conditions. Recombinant Human ATG14 protein has been shown to improve autophagy and reduce disease symptoms in animal models of neurodegenerative diseases.

In addition, recombinant Human ATG14 protein has been studied for its potential use in infectious diseases. Autophagy plays a critical role in host defense against intracellular pathogens, and recombinant Human ATG14 protein has been shown to enhance autophagy and inhibit the growth of various pathogens, including viruses and bacteria. This makes it a promising candidate for the development of novel therapeutics against infectious diseases.

Title: Conclusion

In conclusion, recombinant Human ATG14 protein is a crucial component of the autophagy machinery, playing a key role in autophagy initiation and regulation. Its unique structure and activity make it a promising candidate for various applications, including cancer research, neurodegenerative diseases, and infectious diseases. Further research on this protein may lead to the development of novel therapeutics for these conditions.