Human sCD14 ELISA Kit

Description of Human sCD14 ELISA Kit

The Structure of Human sCD14 ELISA Kit

Human sCD14 ELISA Kit is a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of soluble cluster of differentiation 14 (sCD14) in human serum, plasma, and cell culture supernatants. The kit is composed of a 96-well microplate coated with a monoclonal antibody specific for sCD14, along with a set of standards, controls, and detection reagents.

The sCD14 protein is a glycoprotein with a molecular weight of approximately 55 kDa. It is composed of 375 amino acids and is encoded by the CD14 gene located on chromosome 5q31.1. The protein is predominantly expressed on the surface of monocytes and macrophages, and is also found in smaller amounts on other immune cells such as neutrophils and dendritic cells.

The Activity of Human sCD14 ELISA Kit

The activity of Human sCD14 ELISA Kit is based on the principle of sandwich immunoassay. The microplate is coated with a monoclonal antibody specific for sCD14, which captures the target protein from the sample. After washing away any unbound substances, a biotinylated detection antibody is added, followed by a streptavidin-HRP conjugate. The addition of a substrate solution results in the production of a colorimetric signal, which is directly proportional to the amount of sCD14 present in the sample.

The kit has a sensitivity of 0.1 ng/mL and a dynamic range of 0.78-50 ng/mL, making it a highly sensitive tool for the detection of sCD14. The intra- and inter-assay coefficients of variation (CVs) are less than 10% and 12%, respectively, ensuring accurate and reproducible results.

The Application of Human sCD14 ELISA Kit

Human sCD14 ELISA Kit has a wide range of applications in both research and clinical settings. One of the main applications of this kit is in the study of inflammation and immune response. sCD14 is a key mediator of the innate immune response, playing a crucial role in the recognition of bacterial lipopolysaccharide (LPS) and subsequent activation of the immune system. Therefore, measuring sCD14 levels can provide valuable insights into the inflammatory status of an individual.

In addition, sCD14 has been identified as a potential therapeutic target for various inflammatory diseases, such as sepsis, rheumatoid arthritis, and inflammatory bowel disease. The use of Human sCD14 ELISA Kit can aid in the identification and monitoring of patients who may benefit from therapies targeting sCD14.

Furthermore, the kit can also be used in the diagnosis and monitoring of infectious diseases, as sCD14 levels have been shown to increase in response to viral and bacterial infections. This makes it a valuable tool for the detection and management of diseases such as HIV, tuberculosis, and hepatitis.

In conclusion, Human sCD14 ELISA Kit is a highly sensitive and specific tool for the quantitative measurement of sCD14 in various biological samples. Its wide range of applications in both research and clinical settings make it a valuable asset in the study of inflammation, immune response, and infectious diseases. With its high accuracy and reproducibility, this kit is a reliable choice for researchers and clinicians alike.