Human CDH17/Cadherin-17 HEK293T Stable Cell Line

Introduction

Human CDH17, also known as Cadherin-17, is a transmembrane glycoprotein that belongs to the cadherin family of adhesion molecules. It is expressed in various tissues including the gastrointestinal tract, liver, and pancreas. CDH17 is involved in cell-cell adhesion and plays a crucial role in tissue development, maintenance, and repair. Recent studies have shown that CDH17 is also involved in cancer progression and has emerged as a potential therapeutic target. In this article, we will discuss the structure, activity, and application of the Human CDH17/Cadherin-17 HEK293T Stable Cell Line.

Structure of Human CDH17

The human CDH17 gene is located on chromosome 8q22.1 and consists of 16 exons. The protein encoded by this gene is a type I transmembrane glycoprotein with a molecular weight of approximately 100 kDa. It contains five extracellular cadherin domains, a single transmembrane domain, and a cytoplasmic domain. The extracellular domains of CDH17 are responsible for calcium-dependent homophilic interactions with other cadherin molecules on adjacent cells, while the cytoplasmic domain is involved in intracellular signaling and cytoskeletal interactions.

Activity of Human CDH17

CDH17 is primarily expressed in the gastrointestinal tract, where it plays a role in the maintenance of intestinal epithelial integrity. It is also expressed in the liver and pancreas, where it is involved in tissue development and repair. In addition, CDH17 has been found to be overexpressed in various types of cancer, including colorectal, pancreatic, and gastric cancer. In cancer cells, CDH17 promotes cell migration, invasion, and metastasis by disrupting cell-cell adhesion and promoting epithelial-mesenchymal transition (EMT). CDH17 has also been shown to enhance cancer cell survival and resistance to chemotherapy.

Application of Human CDH17/Cadherin-17 HEK293T Stable Cell Line

The Human CDH17/Cadherin-17 HEK293T Stable Cell Line is a valuable tool for studying the function and regulation of CDH17 in various biological processes, including cancer progression. This cell line is generated by stably transfecting the HEK293T cell line with the human CDH17 gene. The HEK293T cell line is a widely used human embryonic kidney cell line that is easy to culture and has a high transfection efficiency. The stable expression of CDH17 in this cell line allows for the study of its role in cell adhesion, migration, and signaling.

One of the main applications of the Human CDH17/Cadherin-17 HEK293T Stable Cell Line is in flow cytometry. This technique allows for the analysis of cell surface markers and their expression levels on individual cells. The stable expression of CDH17 in this cell line enables the identification and characterization of CDH17-expressing cells, which is particularly useful in cancer research. In addition, the Human CDH17/Cadherin-17 HEK293T Stable Cell Line can be used in co-culture experiments to study the interactions between CDH17-expressing cells and other cell types.

Moreover, the Human CDH17/Cadherin-17 HEK293T Stable Cell Line can be used as a model system for drug discovery and development. As CDH17 is overexpressed in various types of cancer, it has emerged as a potential therapeutic target. The stable expression of CDH17 in this cell line allows for the screening of potential drugs and compounds that can inhibit its activity and potentially be developed as anti-cancer therapies.

Conclusion

In summary, the Human CDH17/Cadherin-17 HEK293T Stable Cell Line is a valuable tool for studying the structure, activity, and application of CDH17 in various biological processes, particularly in cancer research.