Human CD166/ALCAM HEK293T Stable Cell Line

Introduction

Human CD166/ALCAM HEK293T Stable Cell Line is a valuable tool for studying the structure, activity, and potential applications of CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM). This stable cell line is derived from HEK293T cells, a human embryonic kidney cell line commonly used in research and biotechnology. CD166 is a transmembrane glycoprotein that plays a crucial role in cell adhesion and signaling processes. It is expressed in a variety of tissues, including immune cells, endothelial cells, and neural cells, and has been implicated in various diseases such as cancer and inflammatory disorders.

Structure of CD166/ALCAM

CD166/ALCAM is a type I transmembrane protein with a molecular weight of approximately 105 kDa. It consists of a large extracellular domain, a single transmembrane region, and a short cytoplasmic tail. The extracellular domain contains five immunoglobulin-like domains (Ig-like domains) that are involved in cell adhesion and binding to ligands. The cytoplasmic tail contains a conserved motif that interacts with intracellular signaling molecules, allowing CD166 to transmit signals into the cell.

Activity of CD166/ALCAM

CD166/ALCAM is a cell adhesion molecule that mediates interactions between cells and their surrounding environment. It plays a critical role in cell-cell adhesion, cell migration, and tissue development. CD166/ALCAM is also involved in signaling pathways that regulate cell proliferation, survival, and differentiation. The binding of CD166 to its ligands, such as CD6 and CD166 itself, triggers intracellular signaling cascades that modulate cellular functions.

Role of CD166/ALCAM in Cancer

CD166/ALCAM has been implicated in various types of cancer, including breast, lung, and colon cancer. It is overexpressed in many cancer cells and is associated with tumor growth, invasion, and metastasis. CD166/ALCAM promotes cancer cell migration and invasion by interacting with other cell adhesion molecules and extracellular matrix proteins. It also plays a role in the resistance of cancer cells to chemotherapy and radiation therapy.

Role of CD166/ALCAM in Inflammation and Immunity

CD166/ALCAM is expressed on immune cells, such as T cells, B cells, and natural killer cells, and is involved in regulating immune responses. It plays a role in the activation and proliferation of T cells, as well as in the migration of immune cells to sites of inflammation. CD166/ALCAM has also been shown to modulate the function of dendritic cells, which are important for initiating and regulating immune responses.

Applications of CD166/ALCAM HEK293T Stable Cell Line

The CD166/ALCAM HEK293T Stable Cell Line is a valuable tool for studying the role of CD166/ALCAM in various cellular processes and diseases. It can be used to investigate the structure and function of CD166/ALCAM, as well as its interactions with other molecules. The stable cell line can also be used to screen potential therapeutics that target CD166/ALCAM, such as monoclonal antibodies or small molecule inhibitors.

Flow Cytometry Analysis

The CD166/ALCAM HEK293T Stable Cell Line can be used in flow cytometry analysis to quantify the expression of CD166 on the cell surface. This technique allows for the identification and characterization of different cell populations based on their expression of CD166. It can also be used to monitor changes in CD166 expression under different experimental conditions.

Therapeutic Target

CD166/ALCAM has emerged as a potential therapeutic target for various diseases, particularly cancer.