The BasoFlowEx Kit is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation.
Commercially available allergens can be used for the stimulation.
Kit Content:
ED7043-1 Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes.
ED7043-2 Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls.
ED7043-3 Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled.
ED7043-4 Lysing Solution – 30 ml.
Publication
*Rentzos G, Lundberg V, Lundqvist C, Rodrigues R, van Odijk J, Lundell AC, Pullerits T, Telemo E: Use of a basophil activation test as a complementary diagnostic tool in the diagnosis of severe peanut allergy in adults. Clin Transl Allergy. 2015 Jun 11;5:22. doi: 10.1186/s13601-015-0064-9. , URL: https://www.ncbi.nlm.nih.gov/pubmed/?term=Rentzos+G%2C+Lundberg+V%2C+Lundqvist+C
Depince-Berger AE, Sidi-Yahya K, Jeraiby M, Lambert C: Basophil activation test: Implementation and standardization between systems and between instruments. Cytometry A. 2017 Mar;91(3):261-269. , URL: https://www.ncbi.nlm.nih.gov/pubmed/28281330
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