Recombinant Human NUMBL, N-His

Description of Recombinant Human NUMBL, N-His

Introduction

Recombinant Human NUMBL (Numb-like protein) is a protein that plays a crucial role in various cellular processes, including cell differentiation, proliferation, and apoptosis. It belongs to the NUMB family of proteins, which also includes NUMB and NUMB-like 2 (NUMBL2). Recombinant Human NUMBL is a synthetic version of the protein produced through recombinant DNA technology, making it a valuable tool for scientific research and potential therapeutic applications.

Structure of Recombinant Human NUMBL

The gene encoding for Recombinant Human NUMBL is located on chromosome 14 in humans and is composed of 28 exons. The protein itself is composed of 591 amino acids with a molecular weight of approximately 67 kDa. It contains several conserved domains, including a phosphotyrosine-binding (PTB) domain, a proline-rich region, and a C-terminal region rich in serine and threonine residues.

The PTB domain of Recombinant Human NUMBL is responsible for its interaction with other proteins, such as the Notch receptor and the E3 ubiquitin ligase, Itch. The proline-rich region is involved in regulating the stability of the protein, while the C-terminal region is essential for its subcellular localization and activity.

Activity of Recombinant Human NUMBL

Recombinant Human NUMBL has been shown to play a critical role in the Notch signaling pathway, which is essential for cell fate determination and tissue development. It acts as a negative regulator of Notch signaling by binding to the intracellular domain of the Notch receptor and promoting its degradation through the Itch-mediated ubiquitination pathway.

In addition to its role in Notch signaling, Recombinant Human NUMBL has also been implicated in other cellular processes, such as cell cycle regulation, cell migration, and neuronal differentiation. It has been shown to interact with various proteins, including Eps15 homology domain-containing proteins, which are involved in endocytosis and vesicle trafficking, and the tumor suppressor protein p53.

Application of Recombinant Human NUMBL

Due to its crucial role in regulating cellular processes, Recombinant Human NUMBL has become a valuable tool for scientific research. It is commonly used in studies involving the Notch signaling pathway, as well as in investigations of its interaction with other proteins and its impact on various cellular processes.

Furthermore, Recombinant Human NUMBL has potential therapeutic applications. Its involvement in the Notch signaling pathway makes it a potential target for cancer treatment, as dysregulation of this pathway has been linked to various types of cancer. Recombinant Human NUMBL has also been suggested as a potential therapeutic target for neurodegenerative diseases, as it has been shown to play a role in neuronal differentiation and migration.

Conclusion

In summary, Recombinant Human NUMBL is a synthetic version of the protein that plays a crucial role in various cellular processes. Its structure, activity, and potential applications make it a valuable tool for scientific research and a potential target for therapeutic interventions. Further studies on this protein are needed to fully understand its role and potential in various diseases and cellular processes.