HRSV F/Fusion glycoprotein F0 Antigen ELISA Kit

Description of HRSV F/Fusion glycoprotein F0 Antigen ELISA Kit

Introduction to HRSV F/Fusion Glycoprotein F0 Antigen ELISA Kit

Human respiratory syncytial virus (HRSV) is a major cause of respiratory tract infections in infants, young children, and the elderly. It is estimated that HRSV causes 64 million cases of respiratory infections and 160,000 deaths annually worldwide. The fusion (F) glycoprotein is a key antigen in HRSV that plays a critical role in virus entry and infection. Therefore, the detection and measurement of F glycoprotein in clinical samples is crucial for the diagnosis and management of HRSV infections. The HRSV F/Fusion glycoprotein F0 Antigen ELISA Kit is a reliable and sensitive tool for the detection and quantification of F glycoprotein in various biological samples.

Structure of HRSV F/Fusion Glycoprotein F0 Antigen

The HRSV F glycoprotein is a type I transmembrane protein that is synthesized as a precursor protein (F0) of 574 amino acids. The F0 protein is cleaved by host proteases into two subunits, F1 and F2, which are linked by a disulfide bond. The F1 subunit contains the fusion peptide and the heptad repeat regions, while the F2 subunit contains the transmembrane domain and the cytoplasmic tail. The F glycoprotein forms homotrimers on the viral surface, which undergo conformational changes upon binding to the host cell receptor, leading to virus-cell fusion.

Principle of the HRSV F/Fusion Glycoprotein F0 Antigen ELISA Kit

The HRSV F/Fusion glycoprotein F0 Antigen ELISA Kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle. The kit contains pre-coated microplates with monoclonal antibodies specific to the F0 antigen. The F0 antigen in the sample is captured by the coated antibodies, and then a biotinylated detection antibody is added, followed by a streptavidin-HRP conjugate. The HRP enzyme catalyzes a colorimetric reaction, and the intensity of the color is directly proportional to the amount of F0 antigen present in the sample. The absorbance is measured at a wavelength of 450 nm, and the concentration of F0 antigen in the sample is determined by comparing it to a standard curve.

Applications of HRSV F/Fusion Glycoprotein F0 Antigen ELISA Kit

The HRSV F/Fusion glycoprotein F0 Antigen ELISA Kit has multiple applications in the field of HRSV research and diagnosis. It is used for the detection and quantification of F glycoprotein in clinical samples, such as nasopharyngeal swabs, bronchoalveolar lavage fluid, and serum. The kit is also used for screening and monitoring HRSV infections in infants, young children, and immunocompromised patients. Moreover, the HRSV F/Fusion glycoprotein F0 Antigen ELISA Kit is a valuable tool for studying the kinetics of F glycoprotein expression during HRSV infection and the development of neutralizing antibodies.

Therapeutic Target for HRSV Infections

The HRSV F glycoprotein is a potential therapeutic target for the prevention and treatment of HRSV infections. Several studies have shown that neutralizing antibodies targeting the F glycoprotein can inhibit virus entry and fusion, and therefore, prevent infection. The HRSV F/Fusion glycoprotein F0 Antigen ELISA Kit is an essential tool for the development and evaluation of these therapeutic antibodies. It can be used to determine the neutralizing activity of antibodies and to assess their efficacy in clinical trials.

Conclusion

In summary, the HRSV F/Fusion glycoprotein F0 Antigen ELISA Kit is a reliable and sensitive tool for the detection and quantification of F gly