3-5 days if in stock; 3-5 weeks if production needed
Storage condition
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Brand
ExBio
Host species
Mouse
Isotype
IgG1, kappa
Clonality
Monoclonal Antibody
Clone Name
581
Format
Liquid in stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Description of Anti-Hu CD34 PE
The mouse monoclonal antibody 581 reacts with an extracellular epitope of CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.
CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Publication
Sanz E, Muñoz-A N, Monserrat J, Van-Den-Rym A, Escoll P, Ranz I, Alvarez-Mon M, de-la-Hera A: Ordering human CD34+CD10-CD19+ pre/pro-B-cell and CD19- common lymphoid progenitor stages in two pro-B-cell development pathways. Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5925-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/20231472
Goardon N, Nikolousis E, Sternberg A, Chu WK, Craddock C, Richardson P, Benson R, Drayson M, Standen G, Vyas P, Freeman S: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica. 2009 Aug;94(8):1160-3., URL: http://www.ncbi.nlm.nih.gov/pubmed/19644143
Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T: Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction. Circulation. 2006 Mar 14;113(10):1311-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/16534028
Ninos JM, Jefferies LC, Cogle CR, Kerr WG: The thrombopoietin receptor, c-Mpl, is a selective surface marker for human hematopoietic stem cells. J Transl Med. 2006 Feb 16;4:9., URL: https://pubmed.ncbi.nlm.nih.gov/16480521/
Ono F, Sharma BK, Smith CC, Burnett JW, Aurelian L: CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM).J Invest Dermatol. 2005 Jun;124(6):1215-24., URL: https://pubmed.ncbi.nlm.nih.gov/15955097/
Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, and normal bone marrow display different apoptosis and drug resistance-associated phenotypes. Clin Cancer Res. 2004 Nov 15;10(22):7599-606., URL: https://www.ncbi.nlm.nih.gov/pubmed/15569991
Kato S, Ando K, Nakamura Y, Muguruma Y, Sato T, Yabe H, Yabe M, Hattori K, Yasuda Y, Hotta T: Absence of a CD34- hematopoietic precursor population in recipients of CD34+ stem cell transplantation. Bone Marrow Transplant. 2001 Sep;28(6):587-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/11607772
Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ: The adapter protein CrkL associates with CD34. Blood. 2001 Jun 15;97(12):3768-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/11389015
Janowska-Wieczorek A, Marquez LA, Nabholtz JM, Cabuhat ML, Montaño J, Chang H, Rozmus J, Russell JA, Edwards DR, Turner AR: Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane. Blood. 1999 May 15;93(10):3379-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/10233890
Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T: Extensive generation of human cord blood CD34(+) stem cells from Lin(-)CD34(-) cells in a long-term in vitro system. Exp Hematol. 2000 Jun;28(6):690-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/10880755
Bonelli P, Nicolussi P, Manetti R, Antuofermo E, Dattena M: Monoclonal antigen do not cross-react with ovine umbilical cord blood cells. Ital J Anim Sci 2010, vol.9:e6., URL: https://www.tandfonline.com/doi/full/10.4081/ijas.2010.e6
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