Recombinant Human RBM4 Protein, N-His

Description of Recombinant Human RBM4 Protein, N-His

Introduction

Recombinant proteins are essential tools in the field of biotechnology and medical research. These proteins are produced through genetic engineering techniques, where specific DNA sequences are inserted into host cells to produce the desired protein. One such recombinant protein is Recombinant Human RBM4 Protein, which has gained significant attention due to its role in various biological processes. In this article, we will discuss the structure, activity, and application of this protein in detail.

Structure of Recombinant Human RBM4 Protein

Recombinant Human RBM4 Protein is a member of the RNA-binding motif protein family. It is encoded by the RBM4 gene located on chromosome 11 in humans. The protein consists of 374 amino acids and has a molecular weight of approximately 41 kDa. It contains two RNA recognition motifs (RRMs) and a C-terminal arginine-serine-rich (RS) domain. These domains are essential for the protein’s function in RNA binding and splicing regulation.

Activity of Recombinant Human RBM4 Protein

Recombinant Human RBM4 Protein is primarily involved in the regulation of alternative splicing, a process where different combinations of exons are joined together to produce multiple mRNA transcripts from a single gene. This protein binds to specific RNA sequences and influences the splicing of pre-mRNA molecules, thereby controlling the production of different protein isoforms. It has been shown to regulate the splicing of various genes involved in cell growth, differentiation, and apoptosis.

In addition to its role in splicing regulation, Recombinant Human RBM4 Protein has been implicated in other biological processes such as cell cycle progression, RNA stability, and translation. It has also been shown to interact with other proteins involved in RNA splicing, suggesting its involvement in complex regulatory networks.

Application of Recombinant Human RBM4 Protein

The unique structure and activity of Recombinant Human RBM4 Protein make it a valuable tool for various applications in biotechnology and medical research. Some of its applications include:

1. Identification of splicing targets

Recombinant Human RBM4 Protein can be used to identify its RNA binding targets through techniques such as RNA immunoprecipitation (RIP) and cross-linking immunoprecipitation (CLIP). This allows researchers to gain insights into the splicing regulation of specific genes and their role in various biological processes.

2. Development of therapeutic strategies

The dysregulation of alternative splicing has been linked to various diseases, including cancer. Recombinant Human RBM4 Protein can be used to modulate splicing patterns and potentially develop therapeutic strategies for diseases associated with splicing defects.

3. Production of protein isoforms

Recombinant Human RBM4 Protein can be used to produce specific protein isoforms by influencing the splicing of pre-mRNA molecules. This is particularly useful in the production of recombinant proteins with specific functions or properties.

4. Study of RNA splicing mechanisms

Recombinant Human RBM4 Protein has been extensively studied for its role in RNA splicing regulation. Its use in in vitro and in vivo systems has provided valuable insights into the mechanisms of splicing and its regulation by RBM4 and other splicing factors.

5. Diagnostic marker for diseases

The aberrant expression of Recombinant Human RBM4 Protein has been observed in various diseases, including cancer. Its detection in patient samples can serve as a diagnostic marker for disease progression and response to