Question 1

Which antibody discovery technologies do you offer (hybridoma, single B cell, phage display…)?

Accepted Answer

We offer 5 discovery platforms: hybridoma, single B cell, polyclonal, phage display and de novo synthesis. Our extensive and unique phage display libraries such as the world&#8217;s first cancer library, allows us to take on any challenge.

Question 2

How do I know which approach is best for my target or application?

Accepted Answer

Your dedicated PhD expert evaluates your antigen, scientific objectives, application, timeline, and budget to recommend the most appropriate strategy. You receive a tailored plan designed to maximize success and performance in your specific assay.

Question 3

Can you generate antibodies from any host species (mouse, rabbit, camelid, human donors…)?

Accepted Answer

Yes. We can generate antibodies from mouse, rat, rabbit, llama/camelid (VHH), and human donors, and more depending on your project’s goals and constraints.

Question 4

Are you able to handle difficult or low-immunogenic antigens (membrane proteins, toxins, peptides…)?

Accepted Answer

Yes. We can work with any antigen type, including cells, peptides, proteins, small molecules, DNA constructs, VLPs, and nanodiscs. This allows us to successfully address membrane proteins, toxic antigens, highly conserved targets, and low-immunogenic molecules. We offer the strongest guarantees of the market, ensuring you get the solution for your project.

Question 5

What immunization strategies do you propose (peptide, DNA, cell-based, protein…)?

Accepted Answer

We offer peptide, DNA, protein, and cell-based immunization, as well as antigen presentation through VLPs and nanodiscs for complex membrane proteins.Your dedicated PhD expert helps you select the most appropriate strategy based on your antigen type and the performance requirements of your application.

Question 6

Can you deliver recombinant antibodies or hybridoma cell lines depending on my needs?

Accepted Answer

Yes. We can deliver hybridoma cell lines, recombinant antibodies, or both, depending on what best suits your workflow, scalability needs, or long-term production strategy. If needed we can also develop a custom stable cell line.

Question 7

Do you provide sequence information or recombinant reformatting options?

Accepted Answer

Yes. Full sequence information is delivered, and we offer reformatting into any desired format including VHH, Fab, scFv, bispecific formats, Fc fusions, and more.

Question 8

What is the typical timeline for the full antibody generation workflow?

Accepted Answer

Timelines vary depending on the technology, but thanks to our fast-track protocol we can generate your guaranteed custom antibody in less than 4 weeks.

Question 9

How flexible is your workflow, can I start from an antigen, sequence, or even an existing antibody?

Accepted Answer

Our workflows are fully flexible. You can start from an antigen, a target sequence, an existing antibody, a cell line&#8230; We adapt each step based on what you already have.

Question 10

How do you handle intellectual property and confidentiality for custom projects?

Accepted Answer

All custom antibody projects are fully IP-free. You retain complete ownership and exclusive rights to all sequences, antibodies, and cell lines. Confidentiality is protected through NDAs and strict internal procedures.

Question 11

What is your historical success rate with challenging targets?

Accepted Answer

ProteoGenix has a strong track-record for projects involving highly complex or difficult targets. Thanks to our expertise, and extended panel of technology we can solve your toughest challenges. 
 
See an exemple of a complex client&#8217;s project

Question 12

What differentiates your custom antibody generation service from other providers?

Accepted Answer

Our service is fully tailored, IP-free, and built on a wide technology portfolio that covers all antigen types and applications. We can adapt to your timelines and offer a solution for any budget. You benefit from guaranteed performance in your chosen application, multiple Go/No-Go checkpoints, and direct guidance from a dedicated PhD expert.

Question 13

What quality control steps do you perform before delivering the antibody?

Accepted Answer

For recombinant antibody production, our QC workflow systematically includes SDS-PAGE analysis under both reducing and non-reducing conditions.
For custom antibody development, we additionally validate each antibody in the client’s final intended application (e.g., ELISA, IF, WB, etc.), on top of SDS-PAGE (reducing / non-reducing) characterization.

Question 14

Are your antibodies suitable for diagnostic or therapeutic applications?

Accepted Answer

Yes. We generate antibodies for therapeutic discovery, diagnostic development (ELISA, IHC, IF, WB, FC&#8230;), and all research applications. For each field, your project is secured by the strongest guarantees of the market.

Question 15

Can you support antibody optimization, engineering, or humanization after discovery?

Accepted Answer

Yes, our services work in synergy to guide you from antigen design to large-scale production. We can guide you through the entirety of your project. We offer humanization, affinity maturation, isotype switching, Fc engineering, sequence optimization, bispecific design, ADC development, and developability improvement and many more services&#8230;

Question 16

How scalable are your production capabilities, from small test batches to gram-scale?

Accepted Answer

We offer production from microgram-scale for screening to gram-scale manufacturing for preclinical programs.

Question 17

What guarantees do you offer regarding project outcome, quality, or timelines?

Accepted Answer

We offer the strongest guarantees of the market. These guarantees are defined according to your project&#8217;s field. For example, in the case of a diagnostic development project, we guarantee your antibodies performance in your selected application. You can test them in your lab under your own conditions before paying the full price. 
Timelines, quality criteria, and deliverables are clearly defined before project initiation.

Question 18

What kind of post-project support do you provide (additional clones, reformatting, re-expression…)?

Accepted Answer

We offer a comprehensive range of antibody engineering services, including humanization, affinity maturation, bispecific formats, Fc-fusion, and other advanced constructs.
We can also re-express selected clones in various formats such as IgG,  scFv, VHH and more..
If certain antibodies do not meet expectations, we can generate additional clones through secondary screening or subcloning to ensure the best possible outcome.

Custom Antibody Development & Production Services