Question 1

Can you work with any antibody format?

Accepted Answer

Yes. Our custom antibody services are fully adaptable and can be applied to all antibody formats, including IgG, VHH (nanobodies), bispecific antibodies, and other engineered constructs. We tailor the humanization strategy to your molecule’s structure, species origin, and project goals.

Question 2

What does your humanization workflow look like from sequence analysis to final validation?

Accepted Answer

We start by sequencing the parental antibody (hybridoma-derived) and confirming the sequences through analysis. We then express and purify a chimeric version with full QC (SDS-PAGE, WB, UV280) before designing humanized variants using bioinformatics, germline selection, structural modeling, and in silico CDR grafting. Next, we produce at least 18 recombinant humanized variants and validate them through affinity and developability testing, including ELISA, SEC-HPLC (aggregation), and SPR (Biacore/Horiba).

Question 3

How long does a full humanization campaign typically take?

Accepted Answer

A full antibody humanization campaign typically takes 10–15 weeks, depending on the number of variants produced and the extent of functional validation required.Yes. We provide multiple humanized antibody variants (at least 18 purified samples), allowing you to compare candidates and select the best option based on affinity, specificity, and developability.

Question 4

What data packages do you deliver at the end of the project?

Accepted Answer

At the end of the project, we deliver a complete data package including purified humanized antibody variants, the full antibody sequences, and detailed analytical. We provide full recombinant expression, purification, and characterization of the humanized variants, including QC and functional validation assays such as ELISA, SEC-HPLC, and SPR.

Question 5

What in vitro tests do you use to confirm that binding properties are maintained?

Accepted Answer

To confirm that binding properties are maintained, we perform in vitro affinity and kinetics testing using SPR (surface plasmon resonance), providing precise measurements of antibody–antigen interactions.

Question 6

Can you guarantee functional retention after humanization (affinity, specificity, developability)?

Accepted Answer

While functional retention cannot be absolutely guaranteed for every antibody, we strongly secure your project through a no win/no fee commitment and a go/no-go decision points. This allows you to evaluate early functional results before moving forward with full humanization and variant production.

Question 7

Do you provide multiple humanized variants so we can select the best candidate?

Accepted Answer

Yes. We provide multiple humanized antibody variants (at least 18 purified samples), allowing you to compare candidates and select the best option based on affinity, specificity, and developability.

Question 8

What is included in your pricing — sequence analysis only, or full expression and validation?

Accepted Answer

Our pricing is flexible and depends on your project needs. We offer both full humanization packages (including sequence analysis, variant design, recombinant expression, purification, and validation) and customized options, allowing you to select only the services you require.

Question 9

How easily can we transition from humanization to further services such as affinity maturation or stable cell line development?

Accepted Answer

Transitioning is straightforward. From antibody engineering to large-scale production, our services work in full synergy, enabling seamless progression from humanization to affinity maturation services, AI antibody developability optimization platform, or stable cell line development at any stage of your project.

Question 10

Who owns the IP of the humanized sequences?

Accepted Answer

You retain 100% ownership of the IP. All humanized antibody sequences and project results are delivered fully IP-free, with complete freedom for your future development and commercialization.

Monoklonale Antikörper-Humanisierung