Premium flow cytometry guaranteed package: ATX-PACK4M

  • Antigen design (design of the most appropriate fragment to be produced)
  • Two Gene syntheses (each gene optimized for a specific expression system) and two subclonings
  • Expression tests in two systems taken from E. coli, B. Subtilis, P. Pastoris, insect cells, and mammalian cells. To be determined by ProteoGenix. GFP-tagged version also done in parallel to check Flow Cytometry (FC) staining specificity
  • Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
  • Mice injections
  • Immune response control by FACS with transfected cells with GFP obtained at the antigen expression test step
  • Selection of the best two mice for two fusions, in order to have a large number and variety of clones
  • Screening of the fusion product by Flow Cytometry (FC) on transfected cells directly on MoFlo, with cell sorting to separate the best antibody expressing cells.
  • Specificity control using GFP
  • Subcloning of two parental clones, 2-3 rounds of limiting dilutions
  • Production and purification of 2mg from the best clone
  • Level 1 : If we don’t succeed in producing an antibody that detects the immunogen in FC, we charge EUR 1000
  • Level 2 : If we succeed in producing an antibody that works in FC against the immunogen, we charge a part of the cost
  • Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
  • 10 -20 best clones at parental stage for customer’s selection
  • 100 µg of purified antibody to confirm hybridoma purchase
  • 50 µg of purified protein
  After total payment:
  • 1.9 mg of purified antibody
  • One hybridoma cell line (four frozen vials)
  • 150 µg of purified protein
  • Gene in pUC57
  • Gene synthesis: 3-4 weeks
  • Protein production : 7-9 weeks
  • Injections: 8-12 weeks
  • Fusion screening: 5-6 weeks
  • Subcloning: 10-15 weeks
  • Antibody production: 3-4 weeks
  • Total: 36-50weeks
       

PREMIUM Flow cytometry guaranteed package: ATX-PACK4MBis

  • Antigen design (design of the most appropriate fragment to be produced)
  • Two gene syntheses (each gene optimized for a specific expression system) and two subclonings
  • Expression tests in two systems taken from E. coli, B. Subtilis, P. Pastoris, insect cells and mammalian cells. To be determined by ProteoGenix. GFP-tagged version also done in parallel to check FACS staining specificity.
  • Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
  • Mice injections
  • Immune response control by Flow Cytometry (FC) with transfected cells with GFP obtained at antigen expression test step
  • Selection of the two best mice for two fusions, in order to have a large number and variety of clones
  • Screening of the fusion product by ELISA : around 10 x 96-well plates
  • Screening of the positive clones by FACS on transfected cells directly on MoFlo, with cell sorting to separate the best antibody expressing cells.
  • Specificity control using GFP
  • Subcloning of two parental clones 2-3 rounds of limiting dilutions
  • Production and purification of 2mg from the best clone
  • Level 1 : If we don’t succeed in producing an antibody that detects the immunogen in FC, we charge EUR 1 000
  • Level 2 : If we succeed in producing an antibody that works in FC against the immunogen, we charge a part of the cost
  • Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
  • 10 -20 best clones at parental stage for customer’s selection
  • 100 µg of purified antibody to confirm hybridoma purchase
  • 50 µg of purified protein
  After total payment:  
  • 1.9 mg of purified antibody
  • One hybridoma cell line (four frozen vials)
  • 150 µg of purified protein
  • Gene in pUC57
  • Gene synthesis: 3-4 weeks
  • Protein production : 7-9 weeks
  • Injections: 8-12 weeks
  • Fusion screening: 5-6 weeks
  • Subcloning: 8-12 weeks
  • Antibody production: 3-4 weeks
  • Total: 36-50weeks

Request a quote