ProteoGenix has overcome some challenges to be able to offer a unique rabbit monoclonal antibody production service using a premium antibody library and an optimized platform of  antibody phage display for diagnostic applications. These antibodies are particularly adapted to diagnostics due to their enhanced specificity and selectivity, reducing off-target binding and maximizing the success of your projects.

Rabbit monoclonal antibodies are a class of reagents with very high
added value for research, diagnostic and therapeutic applications.

They naturally have:

  • Higher affinity (excellent for IHC usage).
  • Higher specificity (higher signal to noise ratio).
  • Excellent for post translational modification detection.
  • Higher epitope recognition compared to other monoclonal antibodies.
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Rabbit monoclonal antibodies are mostly derived from hybridomas, which are generally not well characterized and which sometimes show high batch-to-batch variability.

The hybridoma method screening is very limited (~103), often it misses rare clones and takes a while to produce antibodies (approximately 9 months). Additionally, clones are often very unstable and antibody production from them can be troublesome. In contrast, phage display combines the superior antigen recognition of the rabbit immune system with the specificity and consistency of a monoclonal antibody generated via hybridoma development without the drawbacks of rabbit hybridomas.

Advantages of rabbit monoclonal antibody production by phage display

Based on their binding affinity and specificity, phage display is one of the most robust technologies used for large-scale studies and the selection of proteins, peptide and antibodies. We believe that monoclonal antibody production in rabbit using phage display offers many advantages compared to other technologies:

Faster and cost effective

We can build phage display libraries very quickly. Our technology will radically reduce the time (3 – 5 months) and expenses for your project

Higher screening range

(107– 109)

Abundance

Once we have created libraries, we can reproduce them promptly in phage and use them for an unlimited number of screenings

Enhance the specificity and stability

with which antibodies recognize antigens in various environmental conditions

Higher production

Of antibody clones at a time

No fusion

No stability issues of rabbit hybridomas and availability of the antibody sequence to secure it forever

Animal protection

As phage display (from naive libraries) doesn’t require any animal use

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ProteoGenix rabbit monoclonal antibody production services content

In this case we offer two possible ways to start the project by phage display:

One method is based on a naive library (coming soon), all the genes of antibodies collected from bone marrow, blood and other samples are included in the library and are suitable for selecting antibodies that match with all kinds of antigens. If you are requesting an immunization with a higher chance of getting the best binders, we offer you the possibility of building an immune library.

Below are the timeframes for the screening of both libraries:

Starting with screening a naive library
Takes about 6-7 weeks (no immunization needed)

Starting with construction and screening an immune library
Takes about 15-18 + 6-7 weeks (including immunization)

Rabbit monoclonal antibody production steps
(Immune library)

Immunization of 3-5 aninimals

Phage display library constrution

Biopanning: screening, amplification and identification

Rabbit Monoclonal Antibody production: Validation by ELISA

For more information or to get a quote for our service, don’t hesitate to contact us.

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