Package details

 
  • Antigen design (design of the most appropriate fragment to be produced)
  • Two gene syntheses (each gene optimized for a specific expression system) and two subclonings
  • Expression tests in two systems taken from E. coli, B. Subtilis, P. Pastoris, insect cells, and mammalian cells. To be determined by ProteoGenix. GFP-tagged version also done in parallel to check IF staining specificity
  • Production of the antigen in the most appropriate expression system, depending on the yield and the project requirements.
  • Mice injections (5 mice)
  • Immune response control by IF with transfected cells with GFP obtained at antigen expression test step.
  • Selection of the best two mice for two fusions in order to have a large number and variety of clones
  • Screening of the fusion product by IF on transfected cells directly
  • Specificity control using GFP
  • Subcloning of two parental clones (2-3 rounds of limiting dilutions)
  • Production and purification of 2mg from the best clone
  • 10 -20 best clones at parental stage for customer’s selection
  • 100 µg of purified antibody to confirm hybridoma purchase
  • 50 µg of purified protein
  After total payment:
  • 1.9 mg of purified antibody
  • One hybridoma cell line (four frozen vials)
  • 150 µg of purified protein
  • Gene in pUC57
  • Level 1 : If we don’t succeed in producing an antibody that detects the immunogen by IF, we charge EUR 1 000
  • Level 2 : If we succeed in producing an antibody that works using IF against the immunogen, we charge a part of the cost
  • Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
  • Gene synthesis: 3-4 weeks
  • Protein production : 7-9 weeks
  • Injections: 8-12 weeks
  • Fusion & screening: 5-6 weeks
  • Subcloning: 10-15 weeks
  • Antibody production: 3-4 weeks
  • Total: 36-50 weeks