Package details
- Two or three peptides design, synthesis and conjugation to KLH
- Mice injections with a mix of the peptides
- Immune response control by ELISA
- Selection of the best mice for fusion
- Screening of the fusion product by ELISA : around 10 x 96-well plates
- Subcloning of 2 parental clones (2-3 rounds of limiting dilutions) examined by Elisa
- Production and purification of 2mg from the best clone
- If we don’t succeed in producing an antibody that works in ELISA against the peptides, we charge EUR 800
- If we succeed, we charge the full package price
- 10 -20 best clones at parental stage for customer’s selection
- One hybridoma cell line (four frozen vials)
- 2 mg of purified antibody
- Peptide synthesis and conjugation : 3-4 weeks
- Injections: 8-12 weeks
- Fusion & screening: 3-4 weeks
- Subcloning: 8-12 weeks
- Antibody production: 3-4 weeks
- Total: 25-36 weeks
Our application guaranteed package list
Standard ELISA: ATX-PACK1M
Premium ELISA: ATX-PACK2M
Premium WB: ATX-PACK3M
Premium Flow Cytometry: ATX-PACK4M
Premium Flow Cytometry: ATX-PACK4MBis
Premium sandwich ELISA: ATX-PACK5M
Premium IF: ATX-PACK6M
Premium IP: ATX-PACK7M
Peptide: ATX-PACK8M
Modification specific: ATX-PACK9M
Premium IHC: ATX-PACK10M