Package details

 
  • Two or three peptides design, synthesis and conjugation to KLH
  • Mice injections with a mix of the peptides
  • Immune response control by ELISA
  • Selection of the best mice for fusion
  • Screening of the fusion product by ELISA : around 10 x 96-well plates
  • Subcloning of 2 parental clones (2-3 rounds of limiting dilutions) examined by Elisa
  • Production and purification of 2mg from the best clone
  • If we don’t succeed in producing an antibody that works in ELISA against the peptides, we charge EUR 800
  • If we succeed, we charge the full package price
  • 10 -20 best clones at parental stage for customer’s selection
  • One hybridoma cell line (four frozen vials)
  • 2 mg of purified antibody
  • Peptide synthesis and conjugation : 3-4 weeks
  • Injections: 8-12 weeks
  • Fusion & screening: 3-4 weeks
  • Subcloning: 8-12 weeks
  • Antibody production: 3-4 weeks
  • Total: 25-36 weeks