Package details

 
  • Synthesis of the peptide with and without modification
  • Mice injections with the modified peptide (5 mice)
  • Immune response control by ELISA (modified peptide)
  • Selection of the best mouse for fusion
  • Positive screening of the fusion product by ELISA with the modified peptide: around 10 x 96-well plates
  • Negative screening with the peptide without the modification
  • Subcloning of two parental clones (2-3 rounds of limiting dilutions) examined by ELISA
  • If we don’t succeed in producing an antibody that works in ELISA against the peptides, we charge EUR 800
  • If we succeed, we charge the full package price
  • 10 -20 best clones at parental stage for customer’s selection
  • One hybridoma cell line (four frozen vials)
  • 2 mg of purified antibody
  • Peptide synthesis and conjugation : 3-4 weeks
  • Injections: 8-12 weeks
  • Fusion & screening: 3-4 weeks
  • Subcloning: 8-12 weeks
  • Antibody production:3-4 weeks
  • Total:25-36 weeks